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Detection of methicillin-resistance gene in Staphylococcus epidermidis strains isolated from patients in Al-Zahra Hospital using polymerase chain reaction and minimum inhibitory concentration methods

BACKGROUND: In recent years, antibiotic resistance of Staphylococcus epidermidis to methicillin has significantly increased, making it essential to study resistance to methicillin, which is a determining factor in the appropriate treatment pattern. The purpose of this study was to identify methicill...

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Autores principales: Pishva, Ebtehaj, Havaei, Seyed Asghar, Arsalani, Firouz, Narimani, Tahmineh, Azimian, Amir, Akbari, Mojtaba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748670/
https://www.ncbi.nlm.nih.gov/pubmed/23977651
http://dx.doi.org/10.4103/2277-9175.108008
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author Pishva, Ebtehaj
Havaei, Seyed Asghar
Arsalani, Firouz
Narimani, Tahmineh
Azimian, Amir
Akbari, Mojtaba
author_facet Pishva, Ebtehaj
Havaei, Seyed Asghar
Arsalani, Firouz
Narimani, Tahmineh
Azimian, Amir
Akbari, Mojtaba
author_sort Pishva, Ebtehaj
collection PubMed
description BACKGROUND: In recent years, antibiotic resistance of Staphylococcus epidermidis to methicillin has significantly increased, making it essential to study resistance to methicillin, which is a determining factor in the appropriate treatment pattern. The purpose of this study was to identify methicillin-resistant genes in S. epidermidis strains using polymerase chain reaction (PCR) and to determine their mean minimum inhibitory concentration (MIC) to methicillin using E-test method. MATERIALS AND METHODS: MIC was determined on 146 samples of S. epidermidis using E-test method. Moreover, all samples were tested for the presence of mecA gene using PCR. RESULTS: PCR test showed 75.34% of the samples to contain mecA gene. Methicillin resistance test was performed using E-test on all the samples, which showed resistance in different dilutions. CONCLUSION: The frequency of mecA gene in S. epidermidis isolates was 75.34%. Among the various applied tests used for determining methicillin resistance, sensitivity and specificity of PCR were the highest and reached 100%. Sensitivity and specificity were found to be 95.3% and 94.7%, respectively, for phenotypic test (E-test) and 86.5% and 80.9%, respectively, for disk diffusion method. Based on the above results, it seems that resistance of S. epidermidis to methicillin is on the rise, and therefore more research is warranted.
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spelling pubmed-37486702013-08-23 Detection of methicillin-resistance gene in Staphylococcus epidermidis strains isolated from patients in Al-Zahra Hospital using polymerase chain reaction and minimum inhibitory concentration methods Pishva, Ebtehaj Havaei, Seyed Asghar Arsalani, Firouz Narimani, Tahmineh Azimian, Amir Akbari, Mojtaba Adv Biomed Res Original Article BACKGROUND: In recent years, antibiotic resistance of Staphylococcus epidermidis to methicillin has significantly increased, making it essential to study resistance to methicillin, which is a determining factor in the appropriate treatment pattern. The purpose of this study was to identify methicillin-resistant genes in S. epidermidis strains using polymerase chain reaction (PCR) and to determine their mean minimum inhibitory concentration (MIC) to methicillin using E-test method. MATERIALS AND METHODS: MIC was determined on 146 samples of S. epidermidis using E-test method. Moreover, all samples were tested for the presence of mecA gene using PCR. RESULTS: PCR test showed 75.34% of the samples to contain mecA gene. Methicillin resistance test was performed using E-test on all the samples, which showed resistance in different dilutions. CONCLUSION: The frequency of mecA gene in S. epidermidis isolates was 75.34%. Among the various applied tests used for determining methicillin resistance, sensitivity and specificity of PCR were the highest and reached 100%. Sensitivity and specificity were found to be 95.3% and 94.7%, respectively, for phenotypic test (E-test) and 86.5% and 80.9%, respectively, for disk diffusion method. Based on the above results, it seems that resistance of S. epidermidis to methicillin is on the rise, and therefore more research is warranted. Medknow Publications & Media Pvt Ltd 2013-03-06 /pmc/articles/PMC3748670/ /pubmed/23977651 http://dx.doi.org/10.4103/2277-9175.108008 Text en Copyright: © 2013 Pishva. http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Article
Pishva, Ebtehaj
Havaei, Seyed Asghar
Arsalani, Firouz
Narimani, Tahmineh
Azimian, Amir
Akbari, Mojtaba
Detection of methicillin-resistance gene in Staphylococcus epidermidis strains isolated from patients in Al-Zahra Hospital using polymerase chain reaction and minimum inhibitory concentration methods
title Detection of methicillin-resistance gene in Staphylococcus epidermidis strains isolated from patients in Al-Zahra Hospital using polymerase chain reaction and minimum inhibitory concentration methods
title_full Detection of methicillin-resistance gene in Staphylococcus epidermidis strains isolated from patients in Al-Zahra Hospital using polymerase chain reaction and minimum inhibitory concentration methods
title_fullStr Detection of methicillin-resistance gene in Staphylococcus epidermidis strains isolated from patients in Al-Zahra Hospital using polymerase chain reaction and minimum inhibitory concentration methods
title_full_unstemmed Detection of methicillin-resistance gene in Staphylococcus epidermidis strains isolated from patients in Al-Zahra Hospital using polymerase chain reaction and minimum inhibitory concentration methods
title_short Detection of methicillin-resistance gene in Staphylococcus epidermidis strains isolated from patients in Al-Zahra Hospital using polymerase chain reaction and minimum inhibitory concentration methods
title_sort detection of methicillin-resistance gene in staphylococcus epidermidis strains isolated from patients in al-zahra hospital using polymerase chain reaction and minimum inhibitory concentration methods
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748670/
https://www.ncbi.nlm.nih.gov/pubmed/23977651
http://dx.doi.org/10.4103/2277-9175.108008
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