Cargando…

Nontargeted Identification of the Phenolic and Other Compounds of Saraca asoca by High Performance Liquid Chromatography-Positive Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry

High performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer was used for separation and identification of phenolic and other compounds in the water extracts of Saraca asoca (Roxb.), De. Wilde. The aim of the study was to identify and evaluate the distribution of p...

Descripción completa

Detalles Bibliográficos
Autores principales: Mittal, Ashwani, Kadyan, Preeti, Gahlaut, Anjum, Dabur, Rajesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748722/
https://www.ncbi.nlm.nih.gov/pubmed/23986876
http://dx.doi.org/10.1155/2013/293935
_version_ 1782281117925113856
author Mittal, Ashwani
Kadyan, Preeti
Gahlaut, Anjum
Dabur, Rajesh
author_facet Mittal, Ashwani
Kadyan, Preeti
Gahlaut, Anjum
Dabur, Rajesh
author_sort Mittal, Ashwani
collection PubMed
description High performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer was used for separation and identification of phenolic and other compounds in the water extracts of Saraca asoca (Roxb.), De. Wilde. The aim of the study was to identify and evaluate the distribution of phenolic compounds in the different parts of the plant. The identity of compounds was established through the comparison with standards and characteristic base peaks as well as other daughter ions. In crude extracts, 34 catechin derivatives, 34 flavonoids, and 17 other compounds were identified. Interestingly, further analysis of compounds showed plant part specific unique pattern of metabolites; that is, regenerated bark is observed to be the best source for catechin/catechin derivative while flowers were found to be the source for wide variety of flavonoids. Moreover, these plant part specific compounds can be used as biomarkers for the identification of plant material or herbal drugs. Overall, the present study provides for the first time a comprehensive analysis of the phenolic components of this herb which may be helpful not only to understand their usage but also to contribute to quality control as well.
format Online
Article
Text
id pubmed-3748722
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-37487222013-08-28 Nontargeted Identification of the Phenolic and Other Compounds of Saraca asoca by High Performance Liquid Chromatography-Positive Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry Mittal, Ashwani Kadyan, Preeti Gahlaut, Anjum Dabur, Rajesh ISRN Pharm Research Article High performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer was used for separation and identification of phenolic and other compounds in the water extracts of Saraca asoca (Roxb.), De. Wilde. The aim of the study was to identify and evaluate the distribution of phenolic compounds in the different parts of the plant. The identity of compounds was established through the comparison with standards and characteristic base peaks as well as other daughter ions. In crude extracts, 34 catechin derivatives, 34 flavonoids, and 17 other compounds were identified. Interestingly, further analysis of compounds showed plant part specific unique pattern of metabolites; that is, regenerated bark is observed to be the best source for catechin/catechin derivative while flowers were found to be the source for wide variety of flavonoids. Moreover, these plant part specific compounds can be used as biomarkers for the identification of plant material or herbal drugs. Overall, the present study provides for the first time a comprehensive analysis of the phenolic components of this herb which may be helpful not only to understand their usage but also to contribute to quality control as well. Hindawi Publishing Corporation 2013-08-05 /pmc/articles/PMC3748722/ /pubmed/23986876 http://dx.doi.org/10.1155/2013/293935 Text en Copyright © 2013 Ashwani Mittal et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mittal, Ashwani
Kadyan, Preeti
Gahlaut, Anjum
Dabur, Rajesh
Nontargeted Identification of the Phenolic and Other Compounds of Saraca asoca by High Performance Liquid Chromatography-Positive Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry
title Nontargeted Identification of the Phenolic and Other Compounds of Saraca asoca by High Performance Liquid Chromatography-Positive Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry
title_full Nontargeted Identification of the Phenolic and Other Compounds of Saraca asoca by High Performance Liquid Chromatography-Positive Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry
title_fullStr Nontargeted Identification of the Phenolic and Other Compounds of Saraca asoca by High Performance Liquid Chromatography-Positive Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry
title_full_unstemmed Nontargeted Identification of the Phenolic and Other Compounds of Saraca asoca by High Performance Liquid Chromatography-Positive Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry
title_short Nontargeted Identification of the Phenolic and Other Compounds of Saraca asoca by High Performance Liquid Chromatography-Positive Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry
title_sort nontargeted identification of the phenolic and other compounds of saraca asoca by high performance liquid chromatography-positive electrospray ionization and quadrupole time-of-flight mass spectrometry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748722/
https://www.ncbi.nlm.nih.gov/pubmed/23986876
http://dx.doi.org/10.1155/2013/293935
work_keys_str_mv AT mittalashwani nontargetedidentificationofthephenolicandothercompoundsofsaracaasocabyhighperformanceliquidchromatographypositiveelectrosprayionizationandquadrupoletimeofflightmassspectrometry
AT kadyanpreeti nontargetedidentificationofthephenolicandothercompoundsofsaracaasocabyhighperformanceliquidchromatographypositiveelectrosprayionizationandquadrupoletimeofflightmassspectrometry
AT gahlautanjum nontargetedidentificationofthephenolicandothercompoundsofsaracaasocabyhighperformanceliquidchromatographypositiveelectrosprayionizationandquadrupoletimeofflightmassspectrometry
AT daburrajesh nontargetedidentificationofthephenolicandothercompoundsofsaracaasocabyhighperformanceliquidchromatographypositiveelectrosprayionizationandquadrupoletimeofflightmassspectrometry