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Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos
Maternal nutrition exclusively during the periconceptional period can induce remarkable effects on both oocyte maturation and early embryo development, which in turn can have lifelong consequences. The objective of this study was to evaluate the effect of maternal methionine supplementation on the t...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749122/ https://www.ncbi.nlm.nih.gov/pubmed/23991086 http://dx.doi.org/10.1371/journal.pone.0072302 |
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author | Peñagaricano, Francisco Souza, Alex H. Carvalho, Paulo D. Driver, Ashley M. Gambra, Rocio Kropp, Jenna Hackbart, Katherine S. Luchini, Daniel Shaver, Randy D. Wiltbank, Milo C. Khatib, Hasan |
author_facet | Peñagaricano, Francisco Souza, Alex H. Carvalho, Paulo D. Driver, Ashley M. Gambra, Rocio Kropp, Jenna Hackbart, Katherine S. Luchini, Daniel Shaver, Randy D. Wiltbank, Milo C. Khatib, Hasan |
author_sort | Peñagaricano, Francisco |
collection | PubMed |
description | Maternal nutrition exclusively during the periconceptional period can induce remarkable effects on both oocyte maturation and early embryo development, which in turn can have lifelong consequences. The objective of this study was to evaluate the effect of maternal methionine supplementation on the transcriptome of bovine preimplantation embryos. Holstein cows were randomly assigned to one of two treatments differing in level of dietary methionine (1.89 Met vs. 2.43 Met % of metabolizable protein) from calving until embryo flushing. High quality preimplantation embryos from individual cows were pooled and then analyzed by RNA sequencing. Remarkably, a subtle difference in methionine supplementation in maternal diet was sufficient to cause significant changes in the transcriptome of the embryos. A total of 276 genes out of 10,662 showed differential expression between treatments (FDR <0.10). Interestingly, several of the most significant genes are related to embryonic development (e.g., VIM, IFI6, BCL2A1, and TBX15) and immune response (e.g., NKG7, TYROBP, SLAMF7, LCP1, and BLA-DQB). Likewise, gene set enrichment analysis revealed that several Gene Ontology terms, InterPro entries, and KEGG pathways were enriched (FDR <0.05) with differentially expressed genes involved in embryo development and immune system. The expression of most genes was decreased by maternal methionine supplementation, consistent with reduced transcription of genes with increased methylation of specific genes by increased methionine. Overall, our findings provide evidence that supplementing methionine to dams prior to conception and during the preimplantation period can modulate gene expression in bovine blastocysts. The ramifications of the observed gene expression changes for subsequent development of the pregnancy and physiology of the offspring warrant further investigation in future studies. |
format | Online Article Text |
id | pubmed-3749122 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37491222013-08-29 Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos Peñagaricano, Francisco Souza, Alex H. Carvalho, Paulo D. Driver, Ashley M. Gambra, Rocio Kropp, Jenna Hackbart, Katherine S. Luchini, Daniel Shaver, Randy D. Wiltbank, Milo C. Khatib, Hasan PLoS One Research Article Maternal nutrition exclusively during the periconceptional period can induce remarkable effects on both oocyte maturation and early embryo development, which in turn can have lifelong consequences. The objective of this study was to evaluate the effect of maternal methionine supplementation on the transcriptome of bovine preimplantation embryos. Holstein cows were randomly assigned to one of two treatments differing in level of dietary methionine (1.89 Met vs. 2.43 Met % of metabolizable protein) from calving until embryo flushing. High quality preimplantation embryos from individual cows were pooled and then analyzed by RNA sequencing. Remarkably, a subtle difference in methionine supplementation in maternal diet was sufficient to cause significant changes in the transcriptome of the embryos. A total of 276 genes out of 10,662 showed differential expression between treatments (FDR <0.10). Interestingly, several of the most significant genes are related to embryonic development (e.g., VIM, IFI6, BCL2A1, and TBX15) and immune response (e.g., NKG7, TYROBP, SLAMF7, LCP1, and BLA-DQB). Likewise, gene set enrichment analysis revealed that several Gene Ontology terms, InterPro entries, and KEGG pathways were enriched (FDR <0.05) with differentially expressed genes involved in embryo development and immune system. The expression of most genes was decreased by maternal methionine supplementation, consistent with reduced transcription of genes with increased methylation of specific genes by increased methionine. Overall, our findings provide evidence that supplementing methionine to dams prior to conception and during the preimplantation period can modulate gene expression in bovine blastocysts. The ramifications of the observed gene expression changes for subsequent development of the pregnancy and physiology of the offspring warrant further investigation in future studies. Public Library of Science 2013-08-21 /pmc/articles/PMC3749122/ /pubmed/23991086 http://dx.doi.org/10.1371/journal.pone.0072302 Text en © 2013 Peñagaricano et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Peñagaricano, Francisco Souza, Alex H. Carvalho, Paulo D. Driver, Ashley M. Gambra, Rocio Kropp, Jenna Hackbart, Katherine S. Luchini, Daniel Shaver, Randy D. Wiltbank, Milo C. Khatib, Hasan Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos |
title | Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos |
title_full | Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos |
title_fullStr | Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos |
title_full_unstemmed | Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos |
title_short | Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos |
title_sort | effect of maternal methionine supplementation on the transcriptome of bovine preimplantation embryos |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749122/ https://www.ncbi.nlm.nih.gov/pubmed/23991086 http://dx.doi.org/10.1371/journal.pone.0072302 |
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