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The Complete Campylobacter jejuni Transcriptome during Colonization of a Natural Host Determined by RNAseq
Campylobacter jejuni is a major human pathogen and a leading cause of bacterial derived gastroenteritis worldwide. C. jejuni regulates gene expression under various environmental conditions and stresses, indicative of its ability to survive in diverse niches. Despite this ability to highly regulate...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749233/ https://www.ncbi.nlm.nih.gov/pubmed/23991199 http://dx.doi.org/10.1371/journal.pone.0073586 |
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author | Taveirne, Michael E. Theriot, Casey M. Livny, Jonathan DiRita, Victor J. |
author_facet | Taveirne, Michael E. Theriot, Casey M. Livny, Jonathan DiRita, Victor J. |
author_sort | Taveirne, Michael E. |
collection | PubMed |
description | Campylobacter jejuni is a major human pathogen and a leading cause of bacterial derived gastroenteritis worldwide. C. jejuni regulates gene expression under various environmental conditions and stresses, indicative of its ability to survive in diverse niches. Despite this ability to highly regulate gene transcription, C. jejuni encodes few transcription factors and its genome lacks many canonical transcriptional regulators. High throughput deep sequencing of mRNA transcripts (termed RNAseq) has been used to study the transcriptome of many different organisms, including C. jejuni; however, this technology has yet to be applied to defining the transcriptome of C. jejuni during in vivo colonization of its natural host, the chicken. In addition to its use in profiling the abundance of annotated genes, RNAseq is a powerful tool for identifying and quantifying, as-of-yet, unknown transcripts including non-coding regulatory RNAs, 5’ untranslated regulatory elements, and anti-sense transcripts. Here we report the complete transcriptome of C. jejuni during colonization of the chicken cecum and in two different in vitro growth phases using strand-specific RNAseq. Through this study, we identified over 250 genes differentially expressed in vivo in addition to numerous putative regulatory RNAs, including trans-acting non-coding RNAs and anti-sense transcripts. These latter potential regulatory elements were not identified in two prior studies using ORF-based microarrays, highlighting the power and value of the RNAseq approach. Our results provide new insights into how C. jejuni responds and adapts to the cecal environment and reveals new functions involved in colonization of its natural host. |
format | Online Article Text |
id | pubmed-3749233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37492332013-08-29 The Complete Campylobacter jejuni Transcriptome during Colonization of a Natural Host Determined by RNAseq Taveirne, Michael E. Theriot, Casey M. Livny, Jonathan DiRita, Victor J. PLoS One Research Article Campylobacter jejuni is a major human pathogen and a leading cause of bacterial derived gastroenteritis worldwide. C. jejuni regulates gene expression under various environmental conditions and stresses, indicative of its ability to survive in diverse niches. Despite this ability to highly regulate gene transcription, C. jejuni encodes few transcription factors and its genome lacks many canonical transcriptional regulators. High throughput deep sequencing of mRNA transcripts (termed RNAseq) has been used to study the transcriptome of many different organisms, including C. jejuni; however, this technology has yet to be applied to defining the transcriptome of C. jejuni during in vivo colonization of its natural host, the chicken. In addition to its use in profiling the abundance of annotated genes, RNAseq is a powerful tool for identifying and quantifying, as-of-yet, unknown transcripts including non-coding regulatory RNAs, 5’ untranslated regulatory elements, and anti-sense transcripts. Here we report the complete transcriptome of C. jejuni during colonization of the chicken cecum and in two different in vitro growth phases using strand-specific RNAseq. Through this study, we identified over 250 genes differentially expressed in vivo in addition to numerous putative regulatory RNAs, including trans-acting non-coding RNAs and anti-sense transcripts. These latter potential regulatory elements were not identified in two prior studies using ORF-based microarrays, highlighting the power and value of the RNAseq approach. Our results provide new insights into how C. jejuni responds and adapts to the cecal environment and reveals new functions involved in colonization of its natural host. Public Library of Science 2013-08-21 /pmc/articles/PMC3749233/ /pubmed/23991199 http://dx.doi.org/10.1371/journal.pone.0073586 Text en © 2013 Taveirne et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Taveirne, Michael E. Theriot, Casey M. Livny, Jonathan DiRita, Victor J. The Complete Campylobacter jejuni Transcriptome during Colonization of a Natural Host Determined by RNAseq |
title | The Complete Campylobacter jejuni Transcriptome during Colonization of a Natural Host Determined by RNAseq |
title_full | The Complete Campylobacter jejuni Transcriptome during Colonization of a Natural Host Determined by RNAseq |
title_fullStr | The Complete Campylobacter jejuni Transcriptome during Colonization of a Natural Host Determined by RNAseq |
title_full_unstemmed | The Complete Campylobacter jejuni Transcriptome during Colonization of a Natural Host Determined by RNAseq |
title_short | The Complete Campylobacter jejuni Transcriptome during Colonization of a Natural Host Determined by RNAseq |
title_sort | complete campylobacter jejuni transcriptome during colonization of a natural host determined by rnaseq |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749233/ https://www.ncbi.nlm.nih.gov/pubmed/23991199 http://dx.doi.org/10.1371/journal.pone.0073586 |
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