An Efficient Method to Prepare PCR Cloning Vectors

An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by further digestion of the small fragment produced by XcmI digestion wit...

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Detalles Bibliográficos
Autores principales: Hong, Soon Gyu, Choi, Ji Young, Pryor, Barry M., Lee, Hong Kum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2009
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749396/
https://www.ncbi.nlm.nih.gov/pubmed/23983541
http://dx.doi.org/10.4489/MYCO.2009.37.3.240
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author Hong, Soon Gyu
Choi, Ji Young
Pryor, Barry M.
Lee, Hong Kum
author_facet Hong, Soon Gyu
Choi, Ji Young
Pryor, Barry M.
Lee, Hong Kum
author_sort Hong, Soon Gyu
collection PubMed
description An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by further digestion of the small fragment produced by XcmI digestion with additional enzymes, and purification with PCR purification kits. Using this procedure, PCR cloning vectors with high ligation efficiencies and low blue or false-positive colonies were obtained.
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spelling pubmed-37493962013-08-27 An Efficient Method to Prepare PCR Cloning Vectors Hong, Soon Gyu Choi, Ji Young Pryor, Barry M. Lee, Hong Kum Mycobiology Research Note An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by further digestion of the small fragment produced by XcmI digestion with additional enzymes, and purification with PCR purification kits. Using this procedure, PCR cloning vectors with high ligation efficiencies and low blue or false-positive colonies were obtained. The Korean Society of Mycology 2009-09 2009-09-30 /pmc/articles/PMC3749396/ /pubmed/23983541 http://dx.doi.org/10.4489/MYCO.2009.37.3.240 Text en Copyright © 2009 The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Note
Hong, Soon Gyu
Choi, Ji Young
Pryor, Barry M.
Lee, Hong Kum
An Efficient Method to Prepare PCR Cloning Vectors
title An Efficient Method to Prepare PCR Cloning Vectors
title_full An Efficient Method to Prepare PCR Cloning Vectors
title_fullStr An Efficient Method to Prepare PCR Cloning Vectors
title_full_unstemmed An Efficient Method to Prepare PCR Cloning Vectors
title_short An Efficient Method to Prepare PCR Cloning Vectors
title_sort efficient method to prepare pcr cloning vectors
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749396/
https://www.ncbi.nlm.nih.gov/pubmed/23983541
http://dx.doi.org/10.4489/MYCO.2009.37.3.240
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