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An Efficient Method to Prepare PCR Cloning Vectors

An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by further digestion of the small fragment produced by XcmI digestion wit...

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Detalles Bibliográficos
Autores principales: Hong, Soon Gyu, Choi, Ji Young, Pryor, Barry M., Lee, Hong Kum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749396/
https://www.ncbi.nlm.nih.gov/pubmed/23983541
http://dx.doi.org/10.4489/MYCO.2009.37.3.240

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