Development of Specific Primer for Tricholoma matsutake

In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR s...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Jang-Han, Han, Yeong-Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2009
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749427/
https://www.ncbi.nlm.nih.gov/pubmed/23983557
http://dx.doi.org/10.4489/MYCO.2009.37.4.317
Descripción
Sumario:In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'-GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study.

Ejemplares similares