Development of Specific Primer for Tricholoma matsutake

In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR s...

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Detalles Bibliográficos
Autores principales: Kim, Jang-Han, Han, Yeong-Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2009
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749427/
https://www.ncbi.nlm.nih.gov/pubmed/23983557
http://dx.doi.org/10.4489/MYCO.2009.37.4.317
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author Kim, Jang-Han
Han, Yeong-Hwan
author_facet Kim, Jang-Han
Han, Yeong-Hwan
author_sort Kim, Jang-Han
collection PubMed
description In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'-GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study.
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spelling pubmed-37494272013-08-27 Development of Specific Primer for Tricholoma matsutake Kim, Jang-Han Han, Yeong-Hwan Mycobiology Research Note In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'-GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study. The Korean Society of Mycology 2009-12 2009-12-31 /pmc/articles/PMC3749427/ /pubmed/23983557 http://dx.doi.org/10.4489/MYCO.2009.37.4.317 Text en Copyright © 2009 The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Note
Kim, Jang-Han
Han, Yeong-Hwan
Development of Specific Primer for Tricholoma matsutake
title Development of Specific Primer for Tricholoma matsutake
title_full Development of Specific Primer for Tricholoma matsutake
title_fullStr Development of Specific Primer for Tricholoma matsutake
title_full_unstemmed Development of Specific Primer for Tricholoma matsutake
title_short Development of Specific Primer for Tricholoma matsutake
title_sort development of specific primer for tricholoma matsutake
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749427/
https://www.ncbi.nlm.nih.gov/pubmed/23983557
http://dx.doi.org/10.4489/MYCO.2009.37.4.317
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