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Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage

Pectobacterium carotovorum subsp. carotovorum is a well-known plant pathogen that causes severe soft rot disease in various crops, resulting in considerable economic loss. To identify pathogenicity-related factors, Chinese cabbage was inoculated with 5314 transposon mutants of P. carotovorum subsp....

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Autores principales: Lee, Dong Hwan, Lim, Jeong-A, Lee, Juneok, Roh, Eunjung, Jung, Kyusuk, Choi, Minseon, Oh, Changsik, Ryu, Sangryeol, Yun, Jongchul, Heu, Sunggi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for General Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749726/
https://www.ncbi.nlm.nih.gov/pubmed/23676432
http://dx.doi.org/10.1099/mic.0.067280-0
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author Lee, Dong Hwan
Lim, Jeong-A
Lee, Juneok
Roh, Eunjung
Jung, Kyusuk
Choi, Minseon
Oh, Changsik
Ryu, Sangryeol
Yun, Jongchul
Heu, Sunggi
author_facet Lee, Dong Hwan
Lim, Jeong-A
Lee, Juneok
Roh, Eunjung
Jung, Kyusuk
Choi, Minseon
Oh, Changsik
Ryu, Sangryeol
Yun, Jongchul
Heu, Sunggi
author_sort Lee, Dong Hwan
collection PubMed
description Pectobacterium carotovorum subsp. carotovorum is a well-known plant pathogen that causes severe soft rot disease in various crops, resulting in considerable economic loss. To identify pathogenicity-related factors, Chinese cabbage was inoculated with 5314 transposon mutants of P. carotovorum subsp. carotovorum Pcc21 derived using Tn5 transposon mutagenesis. A total of 35 reduced-virulence or avirulent mutants were isolated, and 14 loci were identified. The 14 loci could be functionally grouped into nutrient utilization (pyrD, purH, purD, leuA and serB), production of plant cell-wall-degrading enzymes (PCWDEs) (expI, expR and PCC21_023220), motility (flgA, fliA and flhB), biofilm formation (expI, expR and qseC), susceptibility to antibacterial plant chemicals (tolC) and unknown function (ECA2640). Among the 14 genes identified, qseC, tolC and PCC21_023220 are novel pathogenicity factors of P. carotovorum subsp. carotovorum involved in biofilm formation, phytochemical resistance and PCWDE production, respectively.
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spelling pubmed-37497262013-08-28 Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage Lee, Dong Hwan Lim, Jeong-A Lee, Juneok Roh, Eunjung Jung, Kyusuk Choi, Minseon Oh, Changsik Ryu, Sangryeol Yun, Jongchul Heu, Sunggi Microbiology (Reading) Microbial Pathogenicity Pectobacterium carotovorum subsp. carotovorum is a well-known plant pathogen that causes severe soft rot disease in various crops, resulting in considerable economic loss. To identify pathogenicity-related factors, Chinese cabbage was inoculated with 5314 transposon mutants of P. carotovorum subsp. carotovorum Pcc21 derived using Tn5 transposon mutagenesis. A total of 35 reduced-virulence or avirulent mutants were isolated, and 14 loci were identified. The 14 loci could be functionally grouped into nutrient utilization (pyrD, purH, purD, leuA and serB), production of plant cell-wall-degrading enzymes (PCWDEs) (expI, expR and PCC21_023220), motility (flgA, fliA and flhB), biofilm formation (expI, expR and qseC), susceptibility to antibacterial plant chemicals (tolC) and unknown function (ECA2640). Among the 14 genes identified, qseC, tolC and PCC21_023220 are novel pathogenicity factors of P. carotovorum subsp. carotovorum involved in biofilm formation, phytochemical resistance and PCWDE production, respectively. Society for General Microbiology 2013-07 /pmc/articles/PMC3749726/ /pubmed/23676432 http://dx.doi.org/10.1099/mic.0.067280-0 Text en © 2013 SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Microbial Pathogenicity
Lee, Dong Hwan
Lim, Jeong-A
Lee, Juneok
Roh, Eunjung
Jung, Kyusuk
Choi, Minseon
Oh, Changsik
Ryu, Sangryeol
Yun, Jongchul
Heu, Sunggi
Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage
title Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage
title_full Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage
title_fullStr Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage
title_full_unstemmed Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage
title_short Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage
title_sort characterization of genes required for the pathogenicity of pectobacterium carotovorum subsp. carotovorum pcc21 in chinese cabbage
topic Microbial Pathogenicity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749726/
https://www.ncbi.nlm.nih.gov/pubmed/23676432
http://dx.doi.org/10.1099/mic.0.067280-0
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