Cargando…

CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes

BACKGROUND: CIP4 is a scaffold protein that regulates membrane deformation and tubulation, organization of the actin cytoskeleton, endocytosis of growth factor receptors, and vesicle trafficking. Although expressed in the heart, CIP4 has not been studied with regards to its potential function in car...

Descripción completa

Detalles Bibliográficos
Autores principales: Rusconi, Francesca, Thakur, Hrishikesh, Li, Jinliang, Kapiloff, Michael S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3750294/
https://www.ncbi.nlm.nih.gov/pubmed/23915320
http://dx.doi.org/10.1186/1423-0127-20-56
_version_ 1782477096743862272
author Rusconi, Francesca
Thakur, Hrishikesh
Li, Jinliang
Kapiloff, Michael S
author_facet Rusconi, Francesca
Thakur, Hrishikesh
Li, Jinliang
Kapiloff, Michael S
author_sort Rusconi, Francesca
collection PubMed
description BACKGROUND: CIP4 is a scaffold protein that regulates membrane deformation and tubulation, organization of the actin cytoskeleton, endocytosis of growth factor receptors, and vesicle trafficking. Although expressed in the heart, CIP4 has not been studied with regards to its potential function in cardiac myocytes. RESULTS: We now show using RNA interference that CIP4 expression in neonatal rat ventricular myocytes is required for the induction of non-mitotic, hypertrophic growth by the α-adrenergic agonist phenylephrine, the IL-6 cytokine leukemia inhibitor factor, and fetal bovine serum, as assayed using morphometry, immunocytochemistry for the hypertrophic marker atrial natriuretic factor and [(3)H]leucine incorporation for de novo protein synthesis. This requirement was consistent with the induction of CIP4 expression by hypertrophic stimulation. The inhibition of myocyte hypertrophy by CIP4 small interfering oligonucleotides (siRNA) was rescued by expression of a recombinant CIP4 protein, but not by a mutant lacking the N-terminal FCH domain responsible for CIP4 intracellular localization. CONCLUSIONS: These results imply that CIP4 plays a significant role in the intracellular hypertrophic signal transduction network that controls the growth of cardiac myocytes in heart disease.
format Online
Article
Text
id pubmed-3750294
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-37502942013-08-24 CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes Rusconi, Francesca Thakur, Hrishikesh Li, Jinliang Kapiloff, Michael S J Biomed Sci Research BACKGROUND: CIP4 is a scaffold protein that regulates membrane deformation and tubulation, organization of the actin cytoskeleton, endocytosis of growth factor receptors, and vesicle trafficking. Although expressed in the heart, CIP4 has not been studied with regards to its potential function in cardiac myocytes. RESULTS: We now show using RNA interference that CIP4 expression in neonatal rat ventricular myocytes is required for the induction of non-mitotic, hypertrophic growth by the α-adrenergic agonist phenylephrine, the IL-6 cytokine leukemia inhibitor factor, and fetal bovine serum, as assayed using morphometry, immunocytochemistry for the hypertrophic marker atrial natriuretic factor and [(3)H]leucine incorporation for de novo protein synthesis. This requirement was consistent with the induction of CIP4 expression by hypertrophic stimulation. The inhibition of myocyte hypertrophy by CIP4 small interfering oligonucleotides (siRNA) was rescued by expression of a recombinant CIP4 protein, but not by a mutant lacking the N-terminal FCH domain responsible for CIP4 intracellular localization. CONCLUSIONS: These results imply that CIP4 plays a significant role in the intracellular hypertrophic signal transduction network that controls the growth of cardiac myocytes in heart disease. BioMed Central 2013-08-03 /pmc/articles/PMC3750294/ /pubmed/23915320 http://dx.doi.org/10.1186/1423-0127-20-56 Text en Copyright © 2013 Rusconi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Rusconi, Francesca
Thakur, Hrishikesh
Li, Jinliang
Kapiloff, Michael S
CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes
title CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes
title_full CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes
title_fullStr CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes
title_full_unstemmed CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes
title_short CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes
title_sort cip4 is required for the hypertrophic growth of neonatal cardiac myocytes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3750294/
https://www.ncbi.nlm.nih.gov/pubmed/23915320
http://dx.doi.org/10.1186/1423-0127-20-56
work_keys_str_mv AT rusconifrancesca cip4isrequiredforthehypertrophicgrowthofneonatalcardiacmyocytes
AT thakurhrishikesh cip4isrequiredforthehypertrophicgrowthofneonatalcardiacmyocytes
AT lijinliang cip4isrequiredforthehypertrophicgrowthofneonatalcardiacmyocytes
AT kapiloffmichaels cip4isrequiredforthehypertrophicgrowthofneonatalcardiacmyocytes