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Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP

BACKGROUND: Toxoplasma gondii is a widely prevalent protozoan parasite that causes serious toxoplasmosis in humans and animals. The present study aimed to determine the genetic diversity of T. gondii isolates from pigs in Jiangxi, Sichuan, Guangdong Provinces and Chongqing Municipality in China usin...

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Autores principales: Jiang, Hai-Hai, Huang, Si-Yang, Zhou, Dong-Hui, Zhang, Xiao-Xuan, Su, Chunlei, Deng, Shun-Zhou, Zhu, Xing-Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3750303/
https://www.ncbi.nlm.nih.gov/pubmed/23919620
http://dx.doi.org/10.1186/1756-3305-6-227
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author Jiang, Hai-Hai
Huang, Si-Yang
Zhou, Dong-Hui
Zhang, Xiao-Xuan
Su, Chunlei
Deng, Shun-Zhou
Zhu, Xing-Quan
author_facet Jiang, Hai-Hai
Huang, Si-Yang
Zhou, Dong-Hui
Zhang, Xiao-Xuan
Su, Chunlei
Deng, Shun-Zhou
Zhu, Xing-Quan
author_sort Jiang, Hai-Hai
collection PubMed
description BACKGROUND: Toxoplasma gondii is a widely prevalent protozoan parasite that causes serious toxoplasmosis in humans and animals. The present study aimed to determine the genetic diversity of T. gondii isolates from pigs in Jiangxi, Sichuan, Guangdong Provinces and Chongqing Municipality in China using multilocous polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. METHODS: A total of 38 DNA samples were extracted from hilar lymph nodes of pigs with suspected toxoplasmosis, and were detected for the presence of T. gondii by semi-nested PCR of B1 gene. The positive DNA samples were typed at 11 genetic markers, including 10 nuclear loci, namely, SAG1, 5′-SAG2 and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and an apicoplast locus Apico. RESULTS: Twenty-five of the 38 DNA samples were T. gondii B1 gene positive. Complete genotyping data for all loci could be obtained for 17 of the 25 samples. Two genotypes were revealed (ToxoDB PCR-RFLP genotypes #9 and #3). Sixteen samples belong to genotype #9 which is the major lineage in mainland China and one sample belongs to genotype #3 which is Type II variant. CONCLUSIONS: To our knowledge, this is the first report of genetic typing of T. gondii isolates from pigs in Jiangxi, Sichuan Province and Chongqing Municipality, and the first report of ToxoDB #3 T. gondii from pigs in China. These results have implications for the prevention and control of foodborne toxoplasmosis in humans.
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spelling pubmed-37503032013-08-24 Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP Jiang, Hai-Hai Huang, Si-Yang Zhou, Dong-Hui Zhang, Xiao-Xuan Su, Chunlei Deng, Shun-Zhou Zhu, Xing-Quan Parasit Vectors Research BACKGROUND: Toxoplasma gondii is a widely prevalent protozoan parasite that causes serious toxoplasmosis in humans and animals. The present study aimed to determine the genetic diversity of T. gondii isolates from pigs in Jiangxi, Sichuan, Guangdong Provinces and Chongqing Municipality in China using multilocous polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. METHODS: A total of 38 DNA samples were extracted from hilar lymph nodes of pigs with suspected toxoplasmosis, and were detected for the presence of T. gondii by semi-nested PCR of B1 gene. The positive DNA samples were typed at 11 genetic markers, including 10 nuclear loci, namely, SAG1, 5′-SAG2 and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and an apicoplast locus Apico. RESULTS: Twenty-five of the 38 DNA samples were T. gondii B1 gene positive. Complete genotyping data for all loci could be obtained for 17 of the 25 samples. Two genotypes were revealed (ToxoDB PCR-RFLP genotypes #9 and #3). Sixteen samples belong to genotype #9 which is the major lineage in mainland China and one sample belongs to genotype #3 which is Type II variant. CONCLUSIONS: To our knowledge, this is the first report of genetic typing of T. gondii isolates from pigs in Jiangxi, Sichuan Province and Chongqing Municipality, and the first report of ToxoDB #3 T. gondii from pigs in China. These results have implications for the prevention and control of foodborne toxoplasmosis in humans. BioMed Central 2013-08-07 /pmc/articles/PMC3750303/ /pubmed/23919620 http://dx.doi.org/10.1186/1756-3305-6-227 Text en Copyright © 2013 Jiang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Jiang, Hai-Hai
Huang, Si-Yang
Zhou, Dong-Hui
Zhang, Xiao-Xuan
Su, Chunlei
Deng, Shun-Zhou
Zhu, Xing-Quan
Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP
title Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP
title_full Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP
title_fullStr Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP
title_full_unstemmed Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP
title_short Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP
title_sort genetic characterization of toxoplasma gondii from pigs from different localities in china by pcr-rflp
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3750303/
https://www.ncbi.nlm.nih.gov/pubmed/23919620
http://dx.doi.org/10.1186/1756-3305-6-227
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