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Sequencing and validation of housekeeping genes for quantitative real-time PCR during the gonadotrophic cycle of Diploptera punctata

BACKGROUND: Quantitative RT-PCR (q-RT-PCR) is a powerful tool that allows for the large scale analysis of small changes in gene expression. Accurate and reliable results depend on the use of stable reference genes for normalization. However, the expression of some widely used housekeeping genes can...

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Detalles Bibliográficos
Autores principales:	Marchal, Elisabeth, Hult, Ekaterina F, Huang, Juan, Tobe, Stephen S
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3750588/ https://www.ncbi.nlm.nih.gov/pubmed/23777660 http://dx.doi.org/10.1186/1756-0500-6-237

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