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AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris)

BACKGROUND: Calluna vulgaris is one of the most important landscaping plants produced in Germany. Its enormous economic success is due to the prolonged flower attractiveness of mutants in flower morphology, the so-called bud-bloomers. In this study, we present the first genetic linkage map of C. vul...

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Autores principales: Behrend, Anne, Borchert, Thomas, Spiller, Monika, Hohe, Annette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751046/
https://www.ncbi.nlm.nih.gov/pubmed/23915059
http://dx.doi.org/10.1186/1471-2156-14-64
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author Behrend, Anne
Borchert, Thomas
Spiller, Monika
Hohe, Annette
author_facet Behrend, Anne
Borchert, Thomas
Spiller, Monika
Hohe, Annette
author_sort Behrend, Anne
collection PubMed
description BACKGROUND: Calluna vulgaris is one of the most important landscaping plants produced in Germany. Its enormous economic success is due to the prolonged flower attractiveness of mutants in flower morphology, the so-called bud-bloomers. In this study, we present the first genetic linkage map of C. vulgaris in which we mapped a locus of the economically highly desired trait “flower type”. RESULTS: The map was constructed in JoinMap 4.1. using 535 AFLP markers from a single mapping population. A large fraction (40%) of markers showed distorted segregation. To test the effect of segregation distortion on linkage estimation, these markers were sorted regarding their segregation ratio and added in groups to the data set. The plausibility of group formation was evaluated by comparison of the “two-way pseudo-testcross” and the “integrated” mapping approach. Furthermore, regression mapping was compared to the multipoint-likelihood algorithm. The majority of maps constructed by different combinations of these methods consisted of eight linkage groups corresponding to the chromosome number of C. vulgaris. CONCLUSIONS: All maps confirmed the independent inheritance of the most important horticultural traits “flower type”, “flower colour”, and “leaf colour”. An AFLP marker for the most important breeding target “flower type” was identified. The presented genetic map of C. vulgaris can now serve as a basis for further molecular marker selection and map-based cloning of the candidate gene encoding the unique flower architecture of C. vulgaris bud-bloomers.
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spelling pubmed-37510462013-08-24 AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris) Behrend, Anne Borchert, Thomas Spiller, Monika Hohe, Annette BMC Genet Research Article BACKGROUND: Calluna vulgaris is one of the most important landscaping plants produced in Germany. Its enormous economic success is due to the prolonged flower attractiveness of mutants in flower morphology, the so-called bud-bloomers. In this study, we present the first genetic linkage map of C. vulgaris in which we mapped a locus of the economically highly desired trait “flower type”. RESULTS: The map was constructed in JoinMap 4.1. using 535 AFLP markers from a single mapping population. A large fraction (40%) of markers showed distorted segregation. To test the effect of segregation distortion on linkage estimation, these markers were sorted regarding their segregation ratio and added in groups to the data set. The plausibility of group formation was evaluated by comparison of the “two-way pseudo-testcross” and the “integrated” mapping approach. Furthermore, regression mapping was compared to the multipoint-likelihood algorithm. The majority of maps constructed by different combinations of these methods consisted of eight linkage groups corresponding to the chromosome number of C. vulgaris. CONCLUSIONS: All maps confirmed the independent inheritance of the most important horticultural traits “flower type”, “flower colour”, and “leaf colour”. An AFLP marker for the most important breeding target “flower type” was identified. The presented genetic map of C. vulgaris can now serve as a basis for further molecular marker selection and map-based cloning of the candidate gene encoding the unique flower architecture of C. vulgaris bud-bloomers. BioMed Central 2013-08-02 /pmc/articles/PMC3751046/ /pubmed/23915059 http://dx.doi.org/10.1186/1471-2156-14-64 Text en Copyright © 2013 Behrend et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Behrend, Anne
Borchert, Thomas
Spiller, Monika
Hohe, Annette
AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris)
title AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris)
title_full AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris)
title_fullStr AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris)
title_full_unstemmed AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris)
title_short AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris)
title_sort aflp-based genetic mapping of the “bud-flowering” trait in heather (calluna vulgaris)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751046/
https://www.ncbi.nlm.nih.gov/pubmed/23915059
http://dx.doi.org/10.1186/1471-2156-14-64
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