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Protective effects of Scrophularia striata in Ovalbumin-induced mice asthma model

BACKGROUND: Scrophularia striata Boiss. (Scrophulariaceae) is a plant growing in the northeastern part of Iran and being used as a traditional herb for various inflammatory disorders. This study was designed to investigate the protective effects of the Scrophularia striata extract in Ovalbumin (OVA)...

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Autores principales: Azadmehr, Abbas, Hajiaghaee, Reza, Zohal, Mohammad Ali, Maliji, Ghorban
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751247/
https://www.ncbi.nlm.nih.gov/pubmed/23837463
http://dx.doi.org/10.1186/2008-2231-21-56
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author Azadmehr, Abbas
Hajiaghaee, Reza
Zohal, Mohammad Ali
Maliji, Ghorban
author_facet Azadmehr, Abbas
Hajiaghaee, Reza
Zohal, Mohammad Ali
Maliji, Ghorban
author_sort Azadmehr, Abbas
collection PubMed
description BACKGROUND: Scrophularia striata Boiss. (Scrophulariaceae) is a plant growing in the northeastern part of Iran and being used as a traditional herb for various inflammatory disorders. This study was designed to investigate the protective effects of the Scrophularia striata extract in Ovalbumin (OVA) induced-asthma mice model. METHODS: OVA-sensitized mice were intrapritonealy treated with two doses (100 and 200 mg/kg) of the extract on days 8 to 14 separately. Broncoalveolar lavage fluids (BALF) was collected 48 h after the final OVA challenge and then the number of eosinophils and other inflammatory cells were assessed by direct microscopic counting. In addition, total immunoglubolin (Ig) E and OVA-specific IgE levels in serum, IL-4 and IL-5 cytokines in BALF were determined by Enzyme-Linked Immunosorbent Assay. Moreover, phytochemical assay by thin layer chromatography (TLC) and the 2, 2 diphenyl-1-picrylhydrazyl (DPPH) were used to evaluate the main compounds and the antioxidant capacity of the plant extract, respectively. RESULTS: The results showed that the main components; including flavonoids, phenolic compounds and phenyl propanoids were presented in the S. striata extract. In addition, the treatment with extract significantly reduced the number of inflammatory cells and suppressed T-helper 2 (Th2) cytokines including IL-4 and IL-5 in BALF. Also, total IgE and OVA-specific IgE levels in the serum decreased. CONCLUSION: Collectively, it is concluded that the extract has the potential to modulate the Th2 cytokines and could be used as immunomodulatory agent in the treatment of allergic asthma.
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spelling pubmed-37512472013-08-28 Protective effects of Scrophularia striata in Ovalbumin-induced mice asthma model Azadmehr, Abbas Hajiaghaee, Reza Zohal, Mohammad Ali Maliji, Ghorban Daru Research Article BACKGROUND: Scrophularia striata Boiss. (Scrophulariaceae) is a plant growing in the northeastern part of Iran and being used as a traditional herb for various inflammatory disorders. This study was designed to investigate the protective effects of the Scrophularia striata extract in Ovalbumin (OVA) induced-asthma mice model. METHODS: OVA-sensitized mice were intrapritonealy treated with two doses (100 and 200 mg/kg) of the extract on days 8 to 14 separately. Broncoalveolar lavage fluids (BALF) was collected 48 h after the final OVA challenge and then the number of eosinophils and other inflammatory cells were assessed by direct microscopic counting. In addition, total immunoglubolin (Ig) E and OVA-specific IgE levels in serum, IL-4 and IL-5 cytokines in BALF were determined by Enzyme-Linked Immunosorbent Assay. Moreover, phytochemical assay by thin layer chromatography (TLC) and the 2, 2 diphenyl-1-picrylhydrazyl (DPPH) were used to evaluate the main compounds and the antioxidant capacity of the plant extract, respectively. RESULTS: The results showed that the main components; including flavonoids, phenolic compounds and phenyl propanoids were presented in the S. striata extract. In addition, the treatment with extract significantly reduced the number of inflammatory cells and suppressed T-helper 2 (Th2) cytokines including IL-4 and IL-5 in BALF. Also, total IgE and OVA-specific IgE levels in the serum decreased. CONCLUSION: Collectively, it is concluded that the extract has the potential to modulate the Th2 cytokines and could be used as immunomodulatory agent in the treatment of allergic asthma. BioMed Central 2013-07-09 /pmc/articles/PMC3751247/ /pubmed/23837463 http://dx.doi.org/10.1186/2008-2231-21-56 Text en Copyright © 2013 Azadmehr et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Azadmehr, Abbas
Hajiaghaee, Reza
Zohal, Mohammad Ali
Maliji, Ghorban
Protective effects of Scrophularia striata in Ovalbumin-induced mice asthma model
title Protective effects of Scrophularia striata in Ovalbumin-induced mice asthma model
title_full Protective effects of Scrophularia striata in Ovalbumin-induced mice asthma model
title_fullStr Protective effects of Scrophularia striata in Ovalbumin-induced mice asthma model
title_full_unstemmed Protective effects of Scrophularia striata in Ovalbumin-induced mice asthma model
title_short Protective effects of Scrophularia striata in Ovalbumin-induced mice asthma model
title_sort protective effects of scrophularia striata in ovalbumin-induced mice asthma model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751247/
https://www.ncbi.nlm.nih.gov/pubmed/23837463
http://dx.doi.org/10.1186/2008-2231-21-56
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