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Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus

BACKGROUND: In continuing our research into the new family of bifunctional restriction endonucleases (REases), we describe the cloning of the tsoIRM gene. Currently, the family includes six thermostable enzymes: TaqII, Tth111II, TthHB27I, TspGWI, TspDTI, TsoI, isolated from various Thermus sp. and t...

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Detalles Bibliográficos
Autores principales:	Skowron, Piotr M, Vitkute, Jolanta, Ramanauskaite, Danute, Mitkaite, Goda, Jezewska-Frackowiak, Joanna, Zebrowska, Joanna, Zylicz-Stachula, Agnieszka, Lubys, Arvydas
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751577/ https://www.ncbi.nlm.nih.gov/pubmed/23919831 http://dx.doi.org/10.1186/1471-2199-14-17

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