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Genome-wide profiling identifies a subset of methamphetamine (METH)-induced genes associated with METH-induced increased H4K5Ac binding in the rat striatum

BACKGROUND: METH is an illicit drug of abuse that influences gene expression in the rat striatum. Histone modifications regulate gene transcription. METHODS: We therefore used microarray analysis and genome-scale approaches to examine potential relationships between the effects of METH on gene expre...

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Autores principales: Cadet, Jean Lud, Jayanthi, Subramaniam, McCoy, Michael T, Ladenheim, Bruce, Saint-Preux, Fabienne, Lehrmann, Elin, De, Supriyo, Becker, Kevin G, Brannock, Christie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751638/
https://www.ncbi.nlm.nih.gov/pubmed/23937714
http://dx.doi.org/10.1186/1471-2164-14-545
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author Cadet, Jean Lud
Jayanthi, Subramaniam
McCoy, Michael T
Ladenheim, Bruce
Saint-Preux, Fabienne
Lehrmann, Elin
De, Supriyo
Becker, Kevin G
Brannock, Christie
author_facet Cadet, Jean Lud
Jayanthi, Subramaniam
McCoy, Michael T
Ladenheim, Bruce
Saint-Preux, Fabienne
Lehrmann, Elin
De, Supriyo
Becker, Kevin G
Brannock, Christie
author_sort Cadet, Jean Lud
collection PubMed
description BACKGROUND: METH is an illicit drug of abuse that influences gene expression in the rat striatum. Histone modifications regulate gene transcription. METHODS: We therefore used microarray analysis and genome-scale approaches to examine potential relationships between the effects of METH on gene expression and on DNA binding of histone H4 acetylated at lysine 4 (H4K5Ac) in the rat dorsal striatum of METH-naïve and METH-pretreated rats. RESULTS: Acute and chronic METH administration caused differential changes in striatal gene expression. METH also increased H4K5Ac binding around the transcriptional start sites (TSSs) of genes in the rat striatum. In order to relate gene expression to histone acetylation, we binned genes of similar expression into groups of 100 genes and proceeded to relate gene expression to H4K5Ac binding. We found a positive correlation between gene expression and H4K5Ac binding in the striatum of control rats. Similar correlations were observed in METH-treated rats. Genes that showed acute METH-induced increased expression in saline-pretreated rats also showed METH-induced increased H4K5Ac binding. The acute METH injection caused similar increases in H4K5Ac binding in METH-pretreated rats, without affecting gene expression to the same degree. Finally, genes that showed METH-induced decreased expression exhibited either decreases or no changes in H4K5Ac binding. CONCLUSION: Acute METH injections caused increased gene expression of genes that showed increased H4K5Ac binding near their transcription start sites.
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spelling pubmed-37516382013-08-24 Genome-wide profiling identifies a subset of methamphetamine (METH)-induced genes associated with METH-induced increased H4K5Ac binding in the rat striatum Cadet, Jean Lud Jayanthi, Subramaniam McCoy, Michael T Ladenheim, Bruce Saint-Preux, Fabienne Lehrmann, Elin De, Supriyo Becker, Kevin G Brannock, Christie BMC Genomics Research Article BACKGROUND: METH is an illicit drug of abuse that influences gene expression in the rat striatum. Histone modifications regulate gene transcription. METHODS: We therefore used microarray analysis and genome-scale approaches to examine potential relationships between the effects of METH on gene expression and on DNA binding of histone H4 acetylated at lysine 4 (H4K5Ac) in the rat dorsal striatum of METH-naïve and METH-pretreated rats. RESULTS: Acute and chronic METH administration caused differential changes in striatal gene expression. METH also increased H4K5Ac binding around the transcriptional start sites (TSSs) of genes in the rat striatum. In order to relate gene expression to histone acetylation, we binned genes of similar expression into groups of 100 genes and proceeded to relate gene expression to H4K5Ac binding. We found a positive correlation between gene expression and H4K5Ac binding in the striatum of control rats. Similar correlations were observed in METH-treated rats. Genes that showed acute METH-induced increased expression in saline-pretreated rats also showed METH-induced increased H4K5Ac binding. The acute METH injection caused similar increases in H4K5Ac binding in METH-pretreated rats, without affecting gene expression to the same degree. Finally, genes that showed METH-induced decreased expression exhibited either decreases or no changes in H4K5Ac binding. CONCLUSION: Acute METH injections caused increased gene expression of genes that showed increased H4K5Ac binding near their transcription start sites. BioMed Central 2013-08-12 /pmc/articles/PMC3751638/ /pubmed/23937714 http://dx.doi.org/10.1186/1471-2164-14-545 Text en Copyright © 2013 Cadet et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cadet, Jean Lud
Jayanthi, Subramaniam
McCoy, Michael T
Ladenheim, Bruce
Saint-Preux, Fabienne
Lehrmann, Elin
De, Supriyo
Becker, Kevin G
Brannock, Christie
Genome-wide profiling identifies a subset of methamphetamine (METH)-induced genes associated with METH-induced increased H4K5Ac binding in the rat striatum
title Genome-wide profiling identifies a subset of methamphetamine (METH)-induced genes associated with METH-induced increased H4K5Ac binding in the rat striatum
title_full Genome-wide profiling identifies a subset of methamphetamine (METH)-induced genes associated with METH-induced increased H4K5Ac binding in the rat striatum
title_fullStr Genome-wide profiling identifies a subset of methamphetamine (METH)-induced genes associated with METH-induced increased H4K5Ac binding in the rat striatum
title_full_unstemmed Genome-wide profiling identifies a subset of methamphetamine (METH)-induced genes associated with METH-induced increased H4K5Ac binding in the rat striatum
title_short Genome-wide profiling identifies a subset of methamphetamine (METH)-induced genes associated with METH-induced increased H4K5Ac binding in the rat striatum
title_sort genome-wide profiling identifies a subset of methamphetamine (meth)-induced genes associated with meth-induced increased h4k5ac binding in the rat striatum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751638/
https://www.ncbi.nlm.nih.gov/pubmed/23937714
http://dx.doi.org/10.1186/1471-2164-14-545
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