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Kinetics of drug selection systems in mouse embryonic stem cells

BACKGROUND: Stable expression of transgenes is an important technique to analyze gene function. Various drug resistance genes, such as neo, pac, hph, zeo, bsd, and hisD, have been equally used as selection markers to isolate a transfectant without considering their dose-dependent characters. RESULTS...

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Detalles Bibliográficos
Autores principales: Nakatake, Yuhki, Fujii, Setsuko, Masui, Shinji, Sugimoto, Toshimi, Torikai-Nishikawa, Satomi, Adachi, Kenjiro, Niwa, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751694/
https://www.ncbi.nlm.nih.gov/pubmed/23919313
http://dx.doi.org/10.1186/1472-6750-13-64