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Kinetics of drug selection systems in mouse embryonic stem cells
BACKGROUND: Stable expression of transgenes is an important technique to analyze gene function. Various drug resistance genes, such as neo, pac, hph, zeo, bsd, and hisD, have been equally used as selection markers to isolate a transfectant without considering their dose-dependent characters. RESULTS...
Autores principales: | Nakatake, Yuhki, Fujii, Setsuko, Masui, Shinji, Sugimoto, Toshimi, Torikai-Nishikawa, Satomi, Adachi, Kenjiro, Niwa, Hitoshi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751694/ https://www.ncbi.nlm.nih.gov/pubmed/23919313 http://dx.doi.org/10.1186/1472-6750-13-64 |
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