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Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes
INTRODUCTION: Human immunodeficiency virus-1 (HIV-1) infected monocytes are now believed to serve as a reservoir for HIV-1 infection, and to play a role in viral rebound phenomena in certain groups of patients who failed or stopped highly active antiretroviral therapy (HAART). Data characterizing th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3752855/ https://www.ncbi.nlm.nih.gov/pubmed/23984260 http://dx.doi.org/10.4103/2231-0770.112787 |
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author | Gabali, Ali M. Jazaerly, Tarek Cleveland, Ronald Kass, Lawrence |
author_facet | Gabali, Ali M. Jazaerly, Tarek Cleveland, Ronald Kass, Lawrence |
author_sort | Gabali, Ali M. |
collection | PubMed |
description | INTRODUCTION: Human immunodeficiency virus-1 (HIV-1) infected monocytes are now believed to serve as a reservoir for HIV-1 infection, and to play a role in viral rebound phenomena in certain groups of patients who failed or stopped highly active antiretroviral therapy (HAART). Data characterizing the morphological changes of peripheral blood monocytes in HIV-1-infected individuals are limited. MATERIALS AND METHODS: In this study, we collected monocytes from 21 asymptomatic HIV-1-infected individuals with CD4 count more than 500 cells/mm(3) and healthy individuals. The monocytes ultrastructural morphologic changes and α-naphthyl butyrate esterase (ANBE) activity were compared between the two groups. RESULTS: In monocytes from patients infected with HIV-1, activity of α-naphthyl butyrate esterase (ANBE) was markedly increased compared with normal monocytes. In both light microscopic and ultrastructural studies, the cytoplasm of monocytes from HIV-1-infected patients contained a haphazard appearing network of thin fibrils. Cell surface expression of the activation marker HLA-DR molecule was upregulated. There were no discernible differences between the cell surface expression of CD4, CD14, and CD16 molecules comparing normal monocytes to those from HIV-1-infected patients. α-naphthyl butyrate esterase (ANBE) was markedly increased compared with normal monocytes. In both light microscopic and ultrastructural studies, the cytoplasm of monocytes from HIV-1-infected patients contained a haphazard appearing network of thin fibrils. Cell surface expression of the activation marker HLA-DR molecule was upregulated. There were no discernible differences between the cell surface expression of CD4, CD14, and CD16 molecules comparing normal monocytes to those from HIV-1-infected patients. CONCLUSIONS: Possibly, changes in the activity of ANBE along with a disrupted appearing cytoplasmic fibril network contribute to monocyte dysfunction in HIV-1-infected patients. |
format | Online Article Text |
id | pubmed-3752855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-37528552013-08-27 Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes Gabali, Ali M. Jazaerly, Tarek Cleveland, Ronald Kass, Lawrence Avicenna J Med Original Article INTRODUCTION: Human immunodeficiency virus-1 (HIV-1) infected monocytes are now believed to serve as a reservoir for HIV-1 infection, and to play a role in viral rebound phenomena in certain groups of patients who failed or stopped highly active antiretroviral therapy (HAART). Data characterizing the morphological changes of peripheral blood monocytes in HIV-1-infected individuals are limited. MATERIALS AND METHODS: In this study, we collected monocytes from 21 asymptomatic HIV-1-infected individuals with CD4 count more than 500 cells/mm(3) and healthy individuals. The monocytes ultrastructural morphologic changes and α-naphthyl butyrate esterase (ANBE) activity were compared between the two groups. RESULTS: In monocytes from patients infected with HIV-1, activity of α-naphthyl butyrate esterase (ANBE) was markedly increased compared with normal monocytes. In both light microscopic and ultrastructural studies, the cytoplasm of monocytes from HIV-1-infected patients contained a haphazard appearing network of thin fibrils. Cell surface expression of the activation marker HLA-DR molecule was upregulated. There were no discernible differences between the cell surface expression of CD4, CD14, and CD16 molecules comparing normal monocytes to those from HIV-1-infected patients. α-naphthyl butyrate esterase (ANBE) was markedly increased compared with normal monocytes. In both light microscopic and ultrastructural studies, the cytoplasm of monocytes from HIV-1-infected patients contained a haphazard appearing network of thin fibrils. Cell surface expression of the activation marker HLA-DR molecule was upregulated. There were no discernible differences between the cell surface expression of CD4, CD14, and CD16 molecules comparing normal monocytes to those from HIV-1-infected patients. CONCLUSIONS: Possibly, changes in the activity of ANBE along with a disrupted appearing cytoplasmic fibril network contribute to monocyte dysfunction in HIV-1-infected patients. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3752855/ /pubmed/23984260 http://dx.doi.org/10.4103/2231-0770.112787 Text en Copyright: © Avicenna Journal of Medicine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Gabali, Ali M. Jazaerly, Tarek Cleveland, Ronald Kass, Lawrence Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes |
title | Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes |
title_full | Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes |
title_fullStr | Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes |
title_full_unstemmed | Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes |
title_short | Human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes |
title_sort | human immunodeficiency virus type 1 infection alters enzymatic and ultrastructural features of peripheral blood monocytes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3752855/ https://www.ncbi.nlm.nih.gov/pubmed/23984260 http://dx.doi.org/10.4103/2231-0770.112787 |
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