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Ectopic Expression of a WRKY Homolog from Glycine soja Alters Flowering Time in Arabidopsis

Flowering is a critical event in the life cycle of plants; the WRKY-type transcription factors are reported to be involved in many developmental processes sunch as trichome development and epicuticular wax loading, but whether they are involved in flowering time regulation is still unknown. Within t...

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Autores principales: Luo, Xiao, Sun, Xiaoli, Liu, Baohui, Zhu, Dan, Bai, Xi, Cai, Hua, Ji, Wei, Cao, Lei, Wu, Jing, Wang, Mingchao, Ding, Xiaodong, Zhu, Yanming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753250/
https://www.ncbi.nlm.nih.gov/pubmed/23991184
http://dx.doi.org/10.1371/journal.pone.0073295
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author Luo, Xiao
Sun, Xiaoli
Liu, Baohui
Zhu, Dan
Bai, Xi
Cai, Hua
Ji, Wei
Cao, Lei
Wu, Jing
Wang, Mingchao
Ding, Xiaodong
Zhu, Yanming
author_facet Luo, Xiao
Sun, Xiaoli
Liu, Baohui
Zhu, Dan
Bai, Xi
Cai, Hua
Ji, Wei
Cao, Lei
Wu, Jing
Wang, Mingchao
Ding, Xiaodong
Zhu, Yanming
author_sort Luo, Xiao
collection PubMed
description Flowering is a critical event in the life cycle of plants; the WRKY-type transcription factors are reported to be involved in many developmental processes sunch as trichome development and epicuticular wax loading, but whether they are involved in flowering time regulation is still unknown. Within this study, we provide clear evidence that GsWRKY20, a member of WRKY gene family from wild soybean, is involved in controlling plant flowering time. Expression of GsWRKY20 was abundant in the shoot tips and inflorescence meristems of wild soybean. Phenotypic analysis showed that GsWRKY20 over-expression lines flowered earlier than the wild-type plants under all conditions: long-day and short-day photoperiods, vernalization, or exogenous GA(3) application, indicating that GsWRKY20 may mainly be involved in an autonomous flowering pathway. Further analyses by qRT-PCR and microarray suggests that GsWRKY20 accelerating plant flowering might primarily be through the regulation of flowering-related genes (i.e., FLC, FT, SOC1 and CO) and floral meristem identity genes (i.e., AP1, SEP3, AP3, PI and AG). Our results provide the evidence demonstrating the effectiveness of manipulating GsWRKY20 for altering plant flowering time.
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spelling pubmed-37532502013-08-29 Ectopic Expression of a WRKY Homolog from Glycine soja Alters Flowering Time in Arabidopsis Luo, Xiao Sun, Xiaoli Liu, Baohui Zhu, Dan Bai, Xi Cai, Hua Ji, Wei Cao, Lei Wu, Jing Wang, Mingchao Ding, Xiaodong Zhu, Yanming PLoS One Research Article Flowering is a critical event in the life cycle of plants; the WRKY-type transcription factors are reported to be involved in many developmental processes sunch as trichome development and epicuticular wax loading, but whether they are involved in flowering time regulation is still unknown. Within this study, we provide clear evidence that GsWRKY20, a member of WRKY gene family from wild soybean, is involved in controlling plant flowering time. Expression of GsWRKY20 was abundant in the shoot tips and inflorescence meristems of wild soybean. Phenotypic analysis showed that GsWRKY20 over-expression lines flowered earlier than the wild-type plants under all conditions: long-day and short-day photoperiods, vernalization, or exogenous GA(3) application, indicating that GsWRKY20 may mainly be involved in an autonomous flowering pathway. Further analyses by qRT-PCR and microarray suggests that GsWRKY20 accelerating plant flowering might primarily be through the regulation of flowering-related genes (i.e., FLC, FT, SOC1 and CO) and floral meristem identity genes (i.e., AP1, SEP3, AP3, PI and AG). Our results provide the evidence demonstrating the effectiveness of manipulating GsWRKY20 for altering plant flowering time. Public Library of Science 2013-08-26 /pmc/articles/PMC3753250/ /pubmed/23991184 http://dx.doi.org/10.1371/journal.pone.0073295 Text en © 2013 Luo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Luo, Xiao
Sun, Xiaoli
Liu, Baohui
Zhu, Dan
Bai, Xi
Cai, Hua
Ji, Wei
Cao, Lei
Wu, Jing
Wang, Mingchao
Ding, Xiaodong
Zhu, Yanming
Ectopic Expression of a WRKY Homolog from Glycine soja Alters Flowering Time in Arabidopsis
title Ectopic Expression of a WRKY Homolog from Glycine soja Alters Flowering Time in Arabidopsis
title_full Ectopic Expression of a WRKY Homolog from Glycine soja Alters Flowering Time in Arabidopsis
title_fullStr Ectopic Expression of a WRKY Homolog from Glycine soja Alters Flowering Time in Arabidopsis
title_full_unstemmed Ectopic Expression of a WRKY Homolog from Glycine soja Alters Flowering Time in Arabidopsis
title_short Ectopic Expression of a WRKY Homolog from Glycine soja Alters Flowering Time in Arabidopsis
title_sort ectopic expression of a wrky homolog from glycine soja alters flowering time in arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753250/
https://www.ncbi.nlm.nih.gov/pubmed/23991184
http://dx.doi.org/10.1371/journal.pone.0073295
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