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Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM
Mitochondria contain a 16.6 kb circular genome encoding 13 proteins as well as mitochondrial tRNAs and rRNAs. Copies of the genome are organized into nucleoids containing both DNA and proteins, including the machinery required for mtDNA replication and transcription. The transcription factor TFAM is...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753274/ https://www.ncbi.nlm.nih.gov/pubmed/23991223 http://dx.doi.org/10.1371/journal.pone.0074513 |
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author | Wang, Yun E. Marinov, Georgi K. Wold, Barbara J. Chan, David C. |
author_facet | Wang, Yun E. Marinov, Georgi K. Wold, Barbara J. Chan, David C. |
author_sort | Wang, Yun E. |
collection | PubMed |
description | Mitochondria contain a 16.6 kb circular genome encoding 13 proteins as well as mitochondrial tRNAs and rRNAs. Copies of the genome are organized into nucleoids containing both DNA and proteins, including the machinery required for mtDNA replication and transcription. The transcription factor TFAM is critical for initiation of transcription and replication of the genome, and is also thought to perform a packaging function. Although specific binding sites required for initiation of transcription have been identified in the D-loop, little is known about the characteristics of TFAM binding in its nonspecific packaging state. In addition, it is unclear whether TFAM also plays a role in the regulation of nuclear gene expression. Here we investigate these questions by using ChIP-seq to directly localize TFAM binding to DNA in human cells. Our results demonstrate that TFAM uniformly coats the whole mitochondrial genome, with no evidence of robust TFAM binding to the nuclear genome. Our study represents the first high-resolution assessment of TFAM binding on a genome-wide scale in human cells. |
format | Online Article Text |
id | pubmed-3753274 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37532742013-08-29 Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM Wang, Yun E. Marinov, Georgi K. Wold, Barbara J. Chan, David C. PLoS One Research Article Mitochondria contain a 16.6 kb circular genome encoding 13 proteins as well as mitochondrial tRNAs and rRNAs. Copies of the genome are organized into nucleoids containing both DNA and proteins, including the machinery required for mtDNA replication and transcription. The transcription factor TFAM is critical for initiation of transcription and replication of the genome, and is also thought to perform a packaging function. Although specific binding sites required for initiation of transcription have been identified in the D-loop, little is known about the characteristics of TFAM binding in its nonspecific packaging state. In addition, it is unclear whether TFAM also plays a role in the regulation of nuclear gene expression. Here we investigate these questions by using ChIP-seq to directly localize TFAM binding to DNA in human cells. Our results demonstrate that TFAM uniformly coats the whole mitochondrial genome, with no evidence of robust TFAM binding to the nuclear genome. Our study represents the first high-resolution assessment of TFAM binding on a genome-wide scale in human cells. Public Library of Science 2013-08-26 /pmc/articles/PMC3753274/ /pubmed/23991223 http://dx.doi.org/10.1371/journal.pone.0074513 Text en © 2013 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, Yun E. Marinov, Georgi K. Wold, Barbara J. Chan, David C. Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM |
title | Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM |
title_full | Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM |
title_fullStr | Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM |
title_full_unstemmed | Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM |
title_short | Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM |
title_sort | genome-wide analysis reveals coating of the mitochondrial genome by tfam |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753274/ https://www.ncbi.nlm.nih.gov/pubmed/23991223 http://dx.doi.org/10.1371/journal.pone.0074513 |
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