Cargando…

Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM

Mitochondria contain a 16.6 kb circular genome encoding 13 proteins as well as mitochondrial tRNAs and rRNAs. Copies of the genome are organized into nucleoids containing both DNA and proteins, including the machinery required for mtDNA replication and transcription. The transcription factor TFAM is...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yun E., Marinov, Georgi K., Wold, Barbara J., Chan, David C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753274/
https://www.ncbi.nlm.nih.gov/pubmed/23991223
http://dx.doi.org/10.1371/journal.pone.0074513
_version_ 1782281806156922880
author Wang, Yun E.
Marinov, Georgi K.
Wold, Barbara J.
Chan, David C.
author_facet Wang, Yun E.
Marinov, Georgi K.
Wold, Barbara J.
Chan, David C.
author_sort Wang, Yun E.
collection PubMed
description Mitochondria contain a 16.6 kb circular genome encoding 13 proteins as well as mitochondrial tRNAs and rRNAs. Copies of the genome are organized into nucleoids containing both DNA and proteins, including the machinery required for mtDNA replication and transcription. The transcription factor TFAM is critical for initiation of transcription and replication of the genome, and is also thought to perform a packaging function. Although specific binding sites required for initiation of transcription have been identified in the D-loop, little is known about the characteristics of TFAM binding in its nonspecific packaging state. In addition, it is unclear whether TFAM also plays a role in the regulation of nuclear gene expression. Here we investigate these questions by using ChIP-seq to directly localize TFAM binding to DNA in human cells. Our results demonstrate that TFAM uniformly coats the whole mitochondrial genome, with no evidence of robust TFAM binding to the nuclear genome. Our study represents the first high-resolution assessment of TFAM binding on a genome-wide scale in human cells.
format Online
Article
Text
id pubmed-3753274
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-37532742013-08-29 Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM Wang, Yun E. Marinov, Georgi K. Wold, Barbara J. Chan, David C. PLoS One Research Article Mitochondria contain a 16.6 kb circular genome encoding 13 proteins as well as mitochondrial tRNAs and rRNAs. Copies of the genome are organized into nucleoids containing both DNA and proteins, including the machinery required for mtDNA replication and transcription. The transcription factor TFAM is critical for initiation of transcription and replication of the genome, and is also thought to perform a packaging function. Although specific binding sites required for initiation of transcription have been identified in the D-loop, little is known about the characteristics of TFAM binding in its nonspecific packaging state. In addition, it is unclear whether TFAM also plays a role in the regulation of nuclear gene expression. Here we investigate these questions by using ChIP-seq to directly localize TFAM binding to DNA in human cells. Our results demonstrate that TFAM uniformly coats the whole mitochondrial genome, with no evidence of robust TFAM binding to the nuclear genome. Our study represents the first high-resolution assessment of TFAM binding on a genome-wide scale in human cells. Public Library of Science 2013-08-26 /pmc/articles/PMC3753274/ /pubmed/23991223 http://dx.doi.org/10.1371/journal.pone.0074513 Text en © 2013 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Yun E.
Marinov, Georgi K.
Wold, Barbara J.
Chan, David C.
Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM
title Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM
title_full Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM
title_fullStr Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM
title_full_unstemmed Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM
title_short Genome-Wide Analysis Reveals Coating of the Mitochondrial Genome by TFAM
title_sort genome-wide analysis reveals coating of the mitochondrial genome by tfam
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753274/
https://www.ncbi.nlm.nih.gov/pubmed/23991223
http://dx.doi.org/10.1371/journal.pone.0074513
work_keys_str_mv AT wangyune genomewideanalysisrevealscoatingofthemitochondrialgenomebytfam
AT marinovgeorgik genomewideanalysisrevealscoatingofthemitochondrialgenomebytfam
AT woldbarbaraj genomewideanalysisrevealscoatingofthemitochondrialgenomebytfam
AT chandavidc genomewideanalysisrevealscoatingofthemitochondrialgenomebytfam