Quantitative assessment of ratiometric bimolecular beacons as a tool for imaging single engineered RNA transcripts and measuring gene expression in living cells

Recently, we developed an oligonucleotide-based probe, ratiometric bimolecular beacon (RBMB), which generates a detectable fluorescent signal in living cells that express the target RNA. Here, we show that RBMBs can also be used to image single RNA transcripts in living cells, when the target RNA is...

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Detalles Bibliográficos
Autores principales: Zhang, Xuemei, Song, Yang, Shah, Akash Y., Lekova, Virzhiniya, Raj, Arjun, Huang, Ling, Behlke, Mark A., Tsourkas, Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753654/
https://www.ncbi.nlm.nih.gov/pubmed/23814183
http://dx.doi.org/10.1093/nar/gkt561

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753654/
https://www.ncbi.nlm.nih.gov/pubmed/23814183
http://dx.doi.org/10.1093/nar/gkt561

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