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A novel statistical analysis and interpretation of flow cytometry data

A recently developed class of models incorporating the cyton model of population generation structure into a conservation-based model of intracellular label dynamics is reviewed. Statistical aspects of the data collection process are quantified and incorporated into a parameter estimation scheme. Th...

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Autores principales: Banks, H.T., Kapraun, D.F., Thompson, W. Clayton, Peligero, Cristina, Argilaguet, Jordi, Meyerhans, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753657/
https://www.ncbi.nlm.nih.gov/pubmed/23826744
http://dx.doi.org/10.1080/17513758.2013.812753
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author Banks, H.T.
Kapraun, D.F.
Thompson, W. Clayton
Peligero, Cristina
Argilaguet, Jordi
Meyerhans, Andreas
author_facet Banks, H.T.
Kapraun, D.F.
Thompson, W. Clayton
Peligero, Cristina
Argilaguet, Jordi
Meyerhans, Andreas
author_sort Banks, H.T.
collection PubMed
description A recently developed class of models incorporating the cyton model of population generation structure into a conservation-based model of intracellular label dynamics is reviewed. Statistical aspects of the data collection process are quantified and incorporated into a parameter estimation scheme. This scheme is then applied to experimental data for PHA-stimulated CD4+ T and CD8+ T cells collected from two healthy donors. This novel mathematical and statistical framework is shown to form the basis for accurate, meaningful analysis of cellular behaviour for a population of cells labelled with the dye carboxyfluorescein succinimidyl ester and stimulated to divide.
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spelling pubmed-37536572013-08-28 A novel statistical analysis and interpretation of flow cytometry data Banks, H.T. Kapraun, D.F. Thompson, W. Clayton Peligero, Cristina Argilaguet, Jordi Meyerhans, Andreas J Biol Dyn Research Article A recently developed class of models incorporating the cyton model of population generation structure into a conservation-based model of intracellular label dynamics is reviewed. Statistical aspects of the data collection process are quantified and incorporated into a parameter estimation scheme. This scheme is then applied to experimental data for PHA-stimulated CD4+ T and CD8+ T cells collected from two healthy donors. This novel mathematical and statistical framework is shown to form the basis for accurate, meaningful analysis of cellular behaviour for a population of cells labelled with the dye carboxyfluorescein succinimidyl ester and stimulated to divide. Taylor & Francis 2013-07-05 2013-12 /pmc/articles/PMC3753657/ /pubmed/23826744 http://dx.doi.org/10.1080/17513758.2013.812753 Text en © 2013 The Author(s). Published by Taylor & Francis. http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Taylor & Francis journals (http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Banks, H.T.
Kapraun, D.F.
Thompson, W. Clayton
Peligero, Cristina
Argilaguet, Jordi
Meyerhans, Andreas
A novel statistical analysis and interpretation of flow cytometry data
title A novel statistical analysis and interpretation of flow cytometry data
title_full A novel statistical analysis and interpretation of flow cytometry data
title_fullStr A novel statistical analysis and interpretation of flow cytometry data
title_full_unstemmed A novel statistical analysis and interpretation of flow cytometry data
title_short A novel statistical analysis and interpretation of flow cytometry data
title_sort novel statistical analysis and interpretation of flow cytometry data
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753657/
https://www.ncbi.nlm.nih.gov/pubmed/23826744
http://dx.doi.org/10.1080/17513758.2013.812753
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