Cargando…

HMGN1 Modulates Nucleosome Occupancy and DNase I Hypersensitivity at the CpG Island Promoters of Embryonic Stem Cells

Chromatin structure plays a key role in regulating gene expression and embryonic differentiation; however, the factors that determine the organization of chromatin around regulatory sites are not fully known. Here we show that HMGN1, a nucleosome-binding protein ubiquitously expressed in vertebrate...

Descripción completa

Detalles Bibliográficos
Autores principales: Deng, Tao, Zhu, Z. Iris, Zhang, Shaofei, Leng, Fenfei, Cherukuri, Srujana, Hansen, Loren, Mariño-Ramírez, Leonardo, Meshorer, Eran, Landsman, David, Bustin, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753902/
https://www.ncbi.nlm.nih.gov/pubmed/23775126
http://dx.doi.org/10.1128/MCB.00435-13
_version_ 1782281889301659648
author Deng, Tao
Zhu, Z. Iris
Zhang, Shaofei
Leng, Fenfei
Cherukuri, Srujana
Hansen, Loren
Mariño-Ramírez, Leonardo
Meshorer, Eran
Landsman, David
Bustin, Michael
author_facet Deng, Tao
Zhu, Z. Iris
Zhang, Shaofei
Leng, Fenfei
Cherukuri, Srujana
Hansen, Loren
Mariño-Ramírez, Leonardo
Meshorer, Eran
Landsman, David
Bustin, Michael
author_sort Deng, Tao
collection PubMed
description Chromatin structure plays a key role in regulating gene expression and embryonic differentiation; however, the factors that determine the organization of chromatin around regulatory sites are not fully known. Here we show that HMGN1, a nucleosome-binding protein ubiquitously expressed in vertebrate cells, preferentially binds to CpG island-containing promoters and affects the organization of nucleosomes, DNase I hypersensitivity, and the transcriptional profile of mouse embryonic stem cells and neural progenitors. Loss of HMGN1 alters the organization of an unstable nucleosome at transcription start sites, reduces the number of DNase I-hypersensitive sites genome wide, and decreases the number of nestin-positive neural progenitors in the subventricular zone (SVZ) region of mouse brain. Thus, architectural chromatin-binding proteins affect the transcription profile and chromatin structure during embryonic stem cell differentiation.
format Online
Article
Text
id pubmed-3753902
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-37539022013-09-03 HMGN1 Modulates Nucleosome Occupancy and DNase I Hypersensitivity at the CpG Island Promoters of Embryonic Stem Cells Deng, Tao Zhu, Z. Iris Zhang, Shaofei Leng, Fenfei Cherukuri, Srujana Hansen, Loren Mariño-Ramírez, Leonardo Meshorer, Eran Landsman, David Bustin, Michael Mol Cell Biol Articles Chromatin structure plays a key role in regulating gene expression and embryonic differentiation; however, the factors that determine the organization of chromatin around regulatory sites are not fully known. Here we show that HMGN1, a nucleosome-binding protein ubiquitously expressed in vertebrate cells, preferentially binds to CpG island-containing promoters and affects the organization of nucleosomes, DNase I hypersensitivity, and the transcriptional profile of mouse embryonic stem cells and neural progenitors. Loss of HMGN1 alters the organization of an unstable nucleosome at transcription start sites, reduces the number of DNase I-hypersensitive sites genome wide, and decreases the number of nestin-positive neural progenitors in the subventricular zone (SVZ) region of mouse brain. Thus, architectural chromatin-binding proteins affect the transcription profile and chromatin structure during embryonic stem cell differentiation. American Society for Microbiology 2013-08 /pmc/articles/PMC3753902/ /pubmed/23775126 http://dx.doi.org/10.1128/MCB.00435-13 Text en Copyright © 2013, American Society for Microbiology. All Rights Reserved. http://creativecommons.org/licenses/by-nc-sa/3.0/ The authors have paid a fee to allow immediate free access to this article.
spellingShingle Articles
Deng, Tao
Zhu, Z. Iris
Zhang, Shaofei
Leng, Fenfei
Cherukuri, Srujana
Hansen, Loren
Mariño-Ramírez, Leonardo
Meshorer, Eran
Landsman, David
Bustin, Michael
HMGN1 Modulates Nucleosome Occupancy and DNase I Hypersensitivity at the CpG Island Promoters of Embryonic Stem Cells
title HMGN1 Modulates Nucleosome Occupancy and DNase I Hypersensitivity at the CpG Island Promoters of Embryonic Stem Cells
title_full HMGN1 Modulates Nucleosome Occupancy and DNase I Hypersensitivity at the CpG Island Promoters of Embryonic Stem Cells
title_fullStr HMGN1 Modulates Nucleosome Occupancy and DNase I Hypersensitivity at the CpG Island Promoters of Embryonic Stem Cells
title_full_unstemmed HMGN1 Modulates Nucleosome Occupancy and DNase I Hypersensitivity at the CpG Island Promoters of Embryonic Stem Cells
title_short HMGN1 Modulates Nucleosome Occupancy and DNase I Hypersensitivity at the CpG Island Promoters of Embryonic Stem Cells
title_sort hmgn1 modulates nucleosome occupancy and dnase i hypersensitivity at the cpg island promoters of embryonic stem cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753902/
https://www.ncbi.nlm.nih.gov/pubmed/23775126
http://dx.doi.org/10.1128/MCB.00435-13
work_keys_str_mv AT dengtao hmgn1modulatesnucleosomeoccupancyanddnaseihypersensitivityatthecpgislandpromotersofembryonicstemcells
AT zhuziris hmgn1modulatesnucleosomeoccupancyanddnaseihypersensitivityatthecpgislandpromotersofembryonicstemcells
AT zhangshaofei hmgn1modulatesnucleosomeoccupancyanddnaseihypersensitivityatthecpgislandpromotersofembryonicstemcells
AT lengfenfei hmgn1modulatesnucleosomeoccupancyanddnaseihypersensitivityatthecpgislandpromotersofembryonicstemcells
AT cherukurisrujana hmgn1modulatesnucleosomeoccupancyanddnaseihypersensitivityatthecpgislandpromotersofembryonicstemcells
AT hansenloren hmgn1modulatesnucleosomeoccupancyanddnaseihypersensitivityatthecpgislandpromotersofembryonicstemcells
AT marinoramirezleonardo hmgn1modulatesnucleosomeoccupancyanddnaseihypersensitivityatthecpgislandpromotersofembryonicstemcells
AT meshorereran hmgn1modulatesnucleosomeoccupancyanddnaseihypersensitivityatthecpgislandpromotersofembryonicstemcells
AT landsmandavid hmgn1modulatesnucleosomeoccupancyanddnaseihypersensitivityatthecpgislandpromotersofembryonicstemcells
AT bustinmichael hmgn1modulatesnucleosomeoccupancyanddnaseihypersensitivityatthecpgislandpromotersofembryonicstemcells