Uncoupling of Influenza A Virus Transcription and Replication through Mutation of the Unpaired Adenosine in the Viral RNA Promoter

Transcription and replication of the influenza A virus RNA genome are mediated by the viral RNA polymerase from a promoter consisting of the partially base-paired 3′ and 5′ termini of viral genome segments. Here we show that transcription and replication can be uncoupled by mutation of an unpaired a...

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Autores principales: te Velthuis, Aartjan J. W., Turrell, Lauren, Vreede, Frank T., Fodor, Ervin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2013
Materias:
Genome Replication and Regulation of Viral Gene Expression
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753995/
https://www.ncbi.nlm.nih.gov/pubmed/23864616
http://dx.doi.org/10.1128/JVI.00636-13
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author te Velthuis, Aartjan J. W.
Turrell, Lauren
Vreede, Frank T.
Fodor, Ervin
author_facet te Velthuis, Aartjan J. W.
Turrell, Lauren
Vreede, Frank T.
Fodor, Ervin
author_sort te Velthuis, Aartjan J. W.
collection PubMed
description Transcription and replication of the influenza A virus RNA genome are mediated by the viral RNA polymerase from a promoter consisting of the partially base-paired 3′ and 5′ termini of viral genome segments. Here we show that transcription and replication can be uncoupled by mutation of an unpaired adenosine in the 5′ strand of the promoter. This residue is important for transcription but not replication by being essential for the cap-binding activity of the RNA polymerase.
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spelling pubmed-37539952013-09-15 Uncoupling of Influenza A Virus Transcription and Replication through Mutation of the Unpaired Adenosine in the Viral RNA Promoter te Velthuis, Aartjan J. W. Turrell, Lauren Vreede, Frank T. Fodor, Ervin J Virol Genome Replication and Regulation of Viral Gene Expression Transcription and replication of the influenza A virus RNA genome are mediated by the viral RNA polymerase from a promoter consisting of the partially base-paired 3′ and 5′ termini of viral genome segments. Here we show that transcription and replication can be uncoupled by mutation of an unpaired adenosine in the 5′ strand of the promoter. This residue is important for transcription but not replication by being essential for the cap-binding activity of the RNA polymerase. American Society for Microbiology 2013-09 /pmc/articles/PMC3753995/ /pubmed/23864616 http://dx.doi.org/10.1128/JVI.00636-13 Text en Copyright © 2013 te Velthuis et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Genome Replication and Regulation of Viral Gene Expression
te Velthuis, Aartjan J. W.
Turrell, Lauren
Vreede, Frank T.
Fodor, Ervin
Uncoupling of Influenza A Virus Transcription and Replication through Mutation of the Unpaired Adenosine in the Viral RNA Promoter
title Uncoupling of Influenza A Virus Transcription and Replication through Mutation of the Unpaired Adenosine in the Viral RNA Promoter
title_full Uncoupling of Influenza A Virus Transcription and Replication through Mutation of the Unpaired Adenosine in the Viral RNA Promoter
title_fullStr Uncoupling of Influenza A Virus Transcription and Replication through Mutation of the Unpaired Adenosine in the Viral RNA Promoter
title_full_unstemmed Uncoupling of Influenza A Virus Transcription and Replication through Mutation of the Unpaired Adenosine in the Viral RNA Promoter
title_short Uncoupling of Influenza A Virus Transcription and Replication through Mutation of the Unpaired Adenosine in the Viral RNA Promoter
title_sort uncoupling of influenza a virus transcription and replication through mutation of the unpaired adenosine in the viral rna promoter
topic Genome Replication and Regulation of Viral Gene Expression
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753995/
https://www.ncbi.nlm.nih.gov/pubmed/23864616
http://dx.doi.org/10.1128/JVI.00636-13
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