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The Microbial Spectrum of Neonatal Sepsis in Uganda: Recovery of Culturable Bacteria in Mother-Infant Pairs

Neonatal sepsis in the developing world is incompletely characterized. We seek to characterize the microbial spectrum involved in sepsis and determine the role of maternal transmission by comparing organisms that can be cultured from septic newborn infants and their mothers. From 80 consecutive moth...

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Autores principales: Kiwanuka, Julius, Bazira, Joel, Mwanga, Juliet, Tumusiime, Dickson, Nyesigire, Eunice, Lwanga, Nkangi, Warf, Benjamin C., Kapur, Vivek, Poss, Mary, Schiff, Steven J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3754959/
https://www.ncbi.nlm.nih.gov/pubmed/24013829
http://dx.doi.org/10.1371/journal.pone.0072775
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author Kiwanuka, Julius
Bazira, Joel
Mwanga, Juliet
Tumusiime, Dickson
Nyesigire, Eunice
Lwanga, Nkangi
Warf, Benjamin C.
Kapur, Vivek
Poss, Mary
Schiff, Steven J.
author_facet Kiwanuka, Julius
Bazira, Joel
Mwanga, Juliet
Tumusiime, Dickson
Nyesigire, Eunice
Lwanga, Nkangi
Warf, Benjamin C.
Kapur, Vivek
Poss, Mary
Schiff, Steven J.
author_sort Kiwanuka, Julius
collection PubMed
description Neonatal sepsis in the developing world is incompletely characterized. We seek to characterize the microbial spectrum involved in sepsis and determine the role of maternal transmission by comparing organisms that can be cultured from septic newborn infants and their mothers. From 80 consecutive mother-infant pairs meeting clinical criteria for neonatal sepsis, we collected infant blood and spinal fluid, and maternal blood and vaginal specimens. Identifiable bacteria were recovered from the blood in 32.5% of infants, and from 2.5% of cerebrospinal fluid cultures, for a total of 35% recoverable putative causative agents. Bacteria recovered from vaginal specimens were not concordant with those recovered from infants. Similarly there was no concordance of bacteria recovered from blood and cerebrospinal fluid. We conclude that relying on traditional bacterial culture techniques does not adequately delineate the role of maternal versus environmental sources of neonatal sepsis in this setting. More sensitive molecular approaches will be needed to properly characterize the maternal and environmental microbial community involved in neonatal sepsis in such developing countries.
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spelling pubmed-37549592013-09-06 The Microbial Spectrum of Neonatal Sepsis in Uganda: Recovery of Culturable Bacteria in Mother-Infant Pairs Kiwanuka, Julius Bazira, Joel Mwanga, Juliet Tumusiime, Dickson Nyesigire, Eunice Lwanga, Nkangi Warf, Benjamin C. Kapur, Vivek Poss, Mary Schiff, Steven J. PLoS One Research Article Neonatal sepsis in the developing world is incompletely characterized. We seek to characterize the microbial spectrum involved in sepsis and determine the role of maternal transmission by comparing organisms that can be cultured from septic newborn infants and their mothers. From 80 consecutive mother-infant pairs meeting clinical criteria for neonatal sepsis, we collected infant blood and spinal fluid, and maternal blood and vaginal specimens. Identifiable bacteria were recovered from the blood in 32.5% of infants, and from 2.5% of cerebrospinal fluid cultures, for a total of 35% recoverable putative causative agents. Bacteria recovered from vaginal specimens were not concordant with those recovered from infants. Similarly there was no concordance of bacteria recovered from blood and cerebrospinal fluid. We conclude that relying on traditional bacterial culture techniques does not adequately delineate the role of maternal versus environmental sources of neonatal sepsis in this setting. More sensitive molecular approaches will be needed to properly characterize the maternal and environmental microbial community involved in neonatal sepsis in such developing countries. Public Library of Science 2013-08-27 /pmc/articles/PMC3754959/ /pubmed/24013829 http://dx.doi.org/10.1371/journal.pone.0072775 Text en © 2013 Kiwanuka et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kiwanuka, Julius
Bazira, Joel
Mwanga, Juliet
Tumusiime, Dickson
Nyesigire, Eunice
Lwanga, Nkangi
Warf, Benjamin C.
Kapur, Vivek
Poss, Mary
Schiff, Steven J.
The Microbial Spectrum of Neonatal Sepsis in Uganda: Recovery of Culturable Bacteria in Mother-Infant Pairs
title The Microbial Spectrum of Neonatal Sepsis in Uganda: Recovery of Culturable Bacteria in Mother-Infant Pairs
title_full The Microbial Spectrum of Neonatal Sepsis in Uganda: Recovery of Culturable Bacteria in Mother-Infant Pairs
title_fullStr The Microbial Spectrum of Neonatal Sepsis in Uganda: Recovery of Culturable Bacteria in Mother-Infant Pairs
title_full_unstemmed The Microbial Spectrum of Neonatal Sepsis in Uganda: Recovery of Culturable Bacteria in Mother-Infant Pairs
title_short The Microbial Spectrum of Neonatal Sepsis in Uganda: Recovery of Culturable Bacteria in Mother-Infant Pairs
title_sort microbial spectrum of neonatal sepsis in uganda: recovery of culturable bacteria in mother-infant pairs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3754959/
https://www.ncbi.nlm.nih.gov/pubmed/24013829
http://dx.doi.org/10.1371/journal.pone.0072775
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