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Unilateral Hindlimb Casting Induced a Delayed Generalized Muscle Atrophy during Rehabilitation that Is Prevented by a Whey or a High Protein Diet but Not a Free Leucine-Enriched Diet

Sarcopenia is the general muscle mass and strength loss associated with ageing. Muscle atrophy could be made worse by exposure to acute periods of immobilization, because muscle disuse by itself is a stimulus for atrophy. Using a model of unilateral hindlimb casting in old adult rats, we have alread...

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Autores principales: Magne, Hugues, Savary-Auzeloux, Isabelle, Migné, Carole, Peyron, Marie-Agnès, Combaret, Lydie, Rémond, Didier, Dardevet, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3754992/
https://www.ncbi.nlm.nih.gov/pubmed/24015173
http://dx.doi.org/10.1371/journal.pone.0070130
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author Magne, Hugues
Savary-Auzeloux, Isabelle
Migné, Carole
Peyron, Marie-Agnès
Combaret, Lydie
Rémond, Didier
Dardevet, Dominique
author_facet Magne, Hugues
Savary-Auzeloux, Isabelle
Migné, Carole
Peyron, Marie-Agnès
Combaret, Lydie
Rémond, Didier
Dardevet, Dominique
author_sort Magne, Hugues
collection PubMed
description Sarcopenia is the general muscle mass and strength loss associated with ageing. Muscle atrophy could be made worse by exposure to acute periods of immobilization, because muscle disuse by itself is a stimulus for atrophy. Using a model of unilateral hindlimb casting in old adult rats, we have already demonstrated that the primary effect of immobilization was atrophy in the casted leg, but was also surprisingly associated with a retarded atrophy in the non-casted leg during rehabilitation. In search of mechanisms involved in this generalized atrophy, we demonstrated in the present study that contrary to pair-fed non-immobilized control animals, muscle protein synthesis in the non-immobilized limb was unable to adapt and to respond positively to food intake. Because pair-fed control rats did not lose muscle mass, this defect in muscle protein synthesis may represent one of the explanation for the muscle mass loss observed in the non-immobilized rats. Nevertheless, in order to stimulate protein turn over and generate a positive nitrogen balance required to maintain the whole muscle mass in immobilized rats, we tested a dietary free leucine supplementation (an amino acid known for its stimulatory effect on protein metabolism) during the rehabilitation period. Leucine supplementation was able to overcome the anabolic resistance in the non-immobilized limb. A greater muscle protein synthesis up-regulation associated with a stimulation of the mTOR signalling pathway was indeed recorded but it remained inefficient to prevent the loss of muscle in the non-immobilized limb. By contrast, we demonstrated here that whey protein or high protein diets were able to prevent the muscle mass loss of the non-immobilized limb by sustaining muscle protein synthesis during the entire rehabilitation period.
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spelling pubmed-37549922013-09-06 Unilateral Hindlimb Casting Induced a Delayed Generalized Muscle Atrophy during Rehabilitation that Is Prevented by a Whey or a High Protein Diet but Not a Free Leucine-Enriched Diet Magne, Hugues Savary-Auzeloux, Isabelle Migné, Carole Peyron, Marie-Agnès Combaret, Lydie Rémond, Didier Dardevet, Dominique PLoS One Research Article Sarcopenia is the general muscle mass and strength loss associated with ageing. Muscle atrophy could be made worse by exposure to acute periods of immobilization, because muscle disuse by itself is a stimulus for atrophy. Using a model of unilateral hindlimb casting in old adult rats, we have already demonstrated that the primary effect of immobilization was atrophy in the casted leg, but was also surprisingly associated with a retarded atrophy in the non-casted leg during rehabilitation. In search of mechanisms involved in this generalized atrophy, we demonstrated in the present study that contrary to pair-fed non-immobilized control animals, muscle protein synthesis in the non-immobilized limb was unable to adapt and to respond positively to food intake. Because pair-fed control rats did not lose muscle mass, this defect in muscle protein synthesis may represent one of the explanation for the muscle mass loss observed in the non-immobilized rats. Nevertheless, in order to stimulate protein turn over and generate a positive nitrogen balance required to maintain the whole muscle mass in immobilized rats, we tested a dietary free leucine supplementation (an amino acid known for its stimulatory effect on protein metabolism) during the rehabilitation period. Leucine supplementation was able to overcome the anabolic resistance in the non-immobilized limb. A greater muscle protein synthesis up-regulation associated with a stimulation of the mTOR signalling pathway was indeed recorded but it remained inefficient to prevent the loss of muscle in the non-immobilized limb. By contrast, we demonstrated here that whey protein or high protein diets were able to prevent the muscle mass loss of the non-immobilized limb by sustaining muscle protein synthesis during the entire rehabilitation period. Public Library of Science 2013-08-27 /pmc/articles/PMC3754992/ /pubmed/24015173 http://dx.doi.org/10.1371/journal.pone.0070130 Text en © 2013 Magne et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Magne, Hugues
Savary-Auzeloux, Isabelle
Migné, Carole
Peyron, Marie-Agnès
Combaret, Lydie
Rémond, Didier
Dardevet, Dominique
Unilateral Hindlimb Casting Induced a Delayed Generalized Muscle Atrophy during Rehabilitation that Is Prevented by a Whey or a High Protein Diet but Not a Free Leucine-Enriched Diet
title Unilateral Hindlimb Casting Induced a Delayed Generalized Muscle Atrophy during Rehabilitation that Is Prevented by a Whey or a High Protein Diet but Not a Free Leucine-Enriched Diet
title_full Unilateral Hindlimb Casting Induced a Delayed Generalized Muscle Atrophy during Rehabilitation that Is Prevented by a Whey or a High Protein Diet but Not a Free Leucine-Enriched Diet
title_fullStr Unilateral Hindlimb Casting Induced a Delayed Generalized Muscle Atrophy during Rehabilitation that Is Prevented by a Whey or a High Protein Diet but Not a Free Leucine-Enriched Diet
title_full_unstemmed Unilateral Hindlimb Casting Induced a Delayed Generalized Muscle Atrophy during Rehabilitation that Is Prevented by a Whey or a High Protein Diet but Not a Free Leucine-Enriched Diet
title_short Unilateral Hindlimb Casting Induced a Delayed Generalized Muscle Atrophy during Rehabilitation that Is Prevented by a Whey or a High Protein Diet but Not a Free Leucine-Enriched Diet
title_sort unilateral hindlimb casting induced a delayed generalized muscle atrophy during rehabilitation that is prevented by a whey or a high protein diet but not a free leucine-enriched diet
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3754992/
https://www.ncbi.nlm.nih.gov/pubmed/24015173
http://dx.doi.org/10.1371/journal.pone.0070130
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