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Neurotensin Modulates the Migratory and Inflammatory Response of Macrophages under Hyperglycemic Conditions

Diabetic foot ulcers (DFUs) are characterized by an unsatisfactory inflammatory and migratory response. Skin inflammation involves the participation of many cells and particularly macrophages. Macrophage function can be modulated by neuropeptides; however, little is known regarding the role of neuro...

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Autores principales: Moura, Liane I. F., Silva, Lucília, Leal, Ermelindo C., Tellechea, Ana, Cruz, Maria Teresa, Carvalho, Eugénia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3755412/
https://www.ncbi.nlm.nih.gov/pubmed/24000330
http://dx.doi.org/10.1155/2013/941764
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author Moura, Liane I. F.
Silva, Lucília
Leal, Ermelindo C.
Tellechea, Ana
Cruz, Maria Teresa
Carvalho, Eugénia
author_facet Moura, Liane I. F.
Silva, Lucília
Leal, Ermelindo C.
Tellechea, Ana
Cruz, Maria Teresa
Carvalho, Eugénia
author_sort Moura, Liane I. F.
collection PubMed
description Diabetic foot ulcers (DFUs) are characterized by an unsatisfactory inflammatory and migratory response. Skin inflammation involves the participation of many cells and particularly macrophages. Macrophage function can be modulated by neuropeptides; however, little is known regarding the role of neurotensin (NT) as a modulator of macrophages under inflammatory and hyperglycemic conditions. RAW 264.7 cells were maintained at 10/30 mM glucose, stimulated with/without LPS (1 μg/mL), and treated with/without NT(10 nM). The results show that NT did not affect macrophage viability. However, NT reverted the hyperglycemia-induced impair in the migration of macrophages. The expression of IL-6 and IL-1β was significantly increased under 10 mM glucose in the presence of NT, while IL-1β and IL-12 expression significantly decreased under inflammatory and hyperglycemic conditions. More importantly, high glucose modulates NT and NT receptor expression under normal and inflammatory conditions. These results highlight the effect of NT on cell migration, which is strongly impaired under hyperglycemic conditions, as well as its effect in decreasing the proinflammatory status of macrophages under hyperglycemic and inflammatory conditions. These findings provide new insights into the potential therapeutic role of NT in chronic wounds, such as in DFU, characterized by a deficit in the migratory properties of cells and a chronic proinflammatory status.
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spelling pubmed-37554122013-09-02 Neurotensin Modulates the Migratory and Inflammatory Response of Macrophages under Hyperglycemic Conditions Moura, Liane I. F. Silva, Lucília Leal, Ermelindo C. Tellechea, Ana Cruz, Maria Teresa Carvalho, Eugénia Biomed Res Int Research Article Diabetic foot ulcers (DFUs) are characterized by an unsatisfactory inflammatory and migratory response. Skin inflammation involves the participation of many cells and particularly macrophages. Macrophage function can be modulated by neuropeptides; however, little is known regarding the role of neurotensin (NT) as a modulator of macrophages under inflammatory and hyperglycemic conditions. RAW 264.7 cells were maintained at 10/30 mM glucose, stimulated with/without LPS (1 μg/mL), and treated with/without NT(10 nM). The results show that NT did not affect macrophage viability. However, NT reverted the hyperglycemia-induced impair in the migration of macrophages. The expression of IL-6 and IL-1β was significantly increased under 10 mM glucose in the presence of NT, while IL-1β and IL-12 expression significantly decreased under inflammatory and hyperglycemic conditions. More importantly, high glucose modulates NT and NT receptor expression under normal and inflammatory conditions. These results highlight the effect of NT on cell migration, which is strongly impaired under hyperglycemic conditions, as well as its effect in decreasing the proinflammatory status of macrophages under hyperglycemic and inflammatory conditions. These findings provide new insights into the potential therapeutic role of NT in chronic wounds, such as in DFU, characterized by a deficit in the migratory properties of cells and a chronic proinflammatory status. Hindawi Publishing Corporation 2013 2013-08-13 /pmc/articles/PMC3755412/ /pubmed/24000330 http://dx.doi.org/10.1155/2013/941764 Text en Copyright © 2013 Liane I. F. Moura et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Moura, Liane I. F.
Silva, Lucília
Leal, Ermelindo C.
Tellechea, Ana
Cruz, Maria Teresa
Carvalho, Eugénia
Neurotensin Modulates the Migratory and Inflammatory Response of Macrophages under Hyperglycemic Conditions
title Neurotensin Modulates the Migratory and Inflammatory Response of Macrophages under Hyperglycemic Conditions
title_full Neurotensin Modulates the Migratory and Inflammatory Response of Macrophages under Hyperglycemic Conditions
title_fullStr Neurotensin Modulates the Migratory and Inflammatory Response of Macrophages under Hyperglycemic Conditions
title_full_unstemmed Neurotensin Modulates the Migratory and Inflammatory Response of Macrophages under Hyperglycemic Conditions
title_short Neurotensin Modulates the Migratory and Inflammatory Response of Macrophages under Hyperglycemic Conditions
title_sort neurotensin modulates the migratory and inflammatory response of macrophages under hyperglycemic conditions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3755412/
https://www.ncbi.nlm.nih.gov/pubmed/24000330
http://dx.doi.org/10.1155/2013/941764
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