Dynamics of Notch Pathway Expression during Mouse Testis Post-Natal Development and along the Spermatogenic Cycle

The transcription and expression patterns of Notch pathway components (Notch 1–3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle. Notch pat...

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Autores principales: Murta, Daniel, Batista, Marta, Silva, Elisabete, Trindade, Alexandre, Henrique, Domingos, Duarte, António, Lopes-da-Costa, Luís
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3755970/
https://www.ncbi.nlm.nih.gov/pubmed/24015274
http://dx.doi.org/10.1371/journal.pone.0072767
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author Murta, Daniel
Batista, Marta
Silva, Elisabete
Trindade, Alexandre
Henrique, Domingos
Duarte, António
Lopes-da-Costa, Luís
author_facet Murta, Daniel
Batista, Marta
Silva, Elisabete
Trindade, Alexandre
Henrique, Domingos
Duarte, António
Lopes-da-Costa, Luís
author_sort Murta, Daniel
collection PubMed
description The transcription and expression patterns of Notch pathway components (Notch 1–3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle. Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern. This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.
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spelling pubmed-37559702013-09-06 Dynamics of Notch Pathway Expression during Mouse Testis Post-Natal Development and along the Spermatogenic Cycle Murta, Daniel Batista, Marta Silva, Elisabete Trindade, Alexandre Henrique, Domingos Duarte, António Lopes-da-Costa, Luís PLoS One Research Article The transcription and expression patterns of Notch pathway components (Notch 1–3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle. Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern. This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle. Public Library of Science 2013-08-28 /pmc/articles/PMC3755970/ /pubmed/24015274 http://dx.doi.org/10.1371/journal.pone.0072767 Text en © 2013 Murta et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Murta, Daniel
Batista, Marta
Silva, Elisabete
Trindade, Alexandre
Henrique, Domingos
Duarte, António
Lopes-da-Costa, Luís
Dynamics of Notch Pathway Expression during Mouse Testis Post-Natal Development and along the Spermatogenic Cycle
title Dynamics of Notch Pathway Expression during Mouse Testis Post-Natal Development and along the Spermatogenic Cycle
title_full Dynamics of Notch Pathway Expression during Mouse Testis Post-Natal Development and along the Spermatogenic Cycle
title_fullStr Dynamics of Notch Pathway Expression during Mouse Testis Post-Natal Development and along the Spermatogenic Cycle
title_full_unstemmed Dynamics of Notch Pathway Expression during Mouse Testis Post-Natal Development and along the Spermatogenic Cycle
title_short Dynamics of Notch Pathway Expression during Mouse Testis Post-Natal Development and along the Spermatogenic Cycle
title_sort dynamics of notch pathway expression during mouse testis post-natal development and along the spermatogenic cycle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3755970/
https://www.ncbi.nlm.nih.gov/pubmed/24015274
http://dx.doi.org/10.1371/journal.pone.0072767
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