A Single gD Glycoprotein Can Mediate Infection by Herpes simplex Virus

[Image: see text] Herpes simplex viruses display hundreds of gD glycoproteins, and yet their neutralization requires tens of thousands of antibodies per virion, leading us to ask whether a wild-type virion with just a single free gD is still infective. By quantitative analysis of fluorescently label...

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Autores principales: Clarke, Richard W., Drews, Anna, Browne, Helena, Klenerman, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2013
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756529/
https://www.ncbi.nlm.nih.gov/pubmed/23837576
http://dx.doi.org/10.1021/ja4038406
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author Clarke, Richard W.
Drews, Anna
Browne, Helena
Klenerman, David
author_facet Clarke, Richard W.
Drews, Anna
Browne, Helena
Klenerman, David
author_sort Clarke, Richard W.
collection PubMed
description [Image: see text] Herpes simplex viruses display hundreds of gD glycoproteins, and yet their neutralization requires tens of thousands of antibodies per virion, leading us to ask whether a wild-type virion with just a single free gD is still infective. By quantitative analysis of fluorescently labeled virus particles and virus neutralization assays, we show that entry of a wild-type HSV virion to a cell does indeed require just one or two of the approximately 300 gD glycoproteins to be left unbound by monoclonal antibody. This indicates that HSV entry is an extraordinarily efficient process, functioning at the level of single molecular complexes.
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spelling pubmed-37565292013-08-30 A Single gD Glycoprotein Can Mediate Infection by Herpes simplex Virus Clarke, Richard W. Drews, Anna Browne, Helena Klenerman, David J Am Chem Soc [Image: see text] Herpes simplex viruses display hundreds of gD glycoproteins, and yet their neutralization requires tens of thousands of antibodies per virion, leading us to ask whether a wild-type virion with just a single free gD is still infective. By quantitative analysis of fluorescently labeled virus particles and virus neutralization assays, we show that entry of a wild-type HSV virion to a cell does indeed require just one or two of the approximately 300 gD glycoproteins to be left unbound by monoclonal antibody. This indicates that HSV entry is an extraordinarily efficient process, functioning at the level of single molecular complexes. American Chemical Society 2013-07-09 2013-07-31 /pmc/articles/PMC3756529/ /pubmed/23837576 http://dx.doi.org/10.1021/ja4038406 Text en Copyright © 2013 American Chemical Society Terms of Use CC-BY (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html)
spellingShingle Clarke, Richard W.
Drews, Anna
Browne, Helena
Klenerman, David
A Single gD Glycoprotein Can Mediate Infection by Herpes simplex Virus
title A Single gD Glycoprotein Can Mediate Infection by Herpes simplex Virus
title_full A Single gD Glycoprotein Can Mediate Infection by Herpes simplex Virus
title_fullStr A Single gD Glycoprotein Can Mediate Infection by Herpes simplex Virus
title_full_unstemmed A Single gD Glycoprotein Can Mediate Infection by Herpes simplex Virus
title_short A Single gD Glycoprotein Can Mediate Infection by Herpes simplex Virus
title_sort single gd glycoprotein can mediate infection by herpes simplex virus
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756529/
https://www.ncbi.nlm.nih.gov/pubmed/23837576
http://dx.doi.org/10.1021/ja4038406
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