DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips

A photochemical functionalization process which passivates the porous silicon surface of optical biosensors has been optimized as a function of the thickness and the porosity of the devices. The surface modification has been characterized by contact angle measurements. Fluorescence measurements have...

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Detalles Bibliográficos
Autores principales: De Stefano, Luca, Arcari, Paolo, Lamberti, Annalisa, Sanges, Carmen, Rotiroti, Lucia, Rea, Ilaria, Rendina, Ivo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2007
Materias:
Full Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756702/
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author De Stefano, Luca
Arcari, Paolo
Lamberti, Annalisa
Sanges, Carmen
Rotiroti, Lucia
Rea, Ilaria
Rendina, Ivo
author_facet De Stefano, Luca
Arcari, Paolo
Lamberti, Annalisa
Sanges, Carmen
Rotiroti, Lucia
Rea, Ilaria
Rendina, Ivo
author_sort De Stefano, Luca
collection PubMed
description A photochemical functionalization process which passivates the porous silicon surface of optical biosensors has been optimized as a function of the thickness and the porosity of the devices. The surface modification has been characterized by contact angle measurements. Fluorescence measurements have been used to investigate the stability of the DNA single strands bound to the nanostructured material. A dose-response curve for an optical label-free biosensor in the 6-80 mM range has been realized.
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spelling pubmed-37567022013-08-29 DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips De Stefano, Luca Arcari, Paolo Lamberti, Annalisa Sanges, Carmen Rotiroti, Lucia Rea, Ilaria Rendina, Ivo Sensors (Basel) Full Research Paper A photochemical functionalization process which passivates the porous silicon surface of optical biosensors has been optimized as a function of the thickness and the porosity of the devices. The surface modification has been characterized by contact angle measurements. Fluorescence measurements have been used to investigate the stability of the DNA single strands bound to the nanostructured material. A dose-response curve for an optical label-free biosensor in the 6-80 mM range has been realized. Molecular Diversity Preservation International (MDPI) 2007-02-28 /pmc/articles/PMC3756702/ Text en © 2007 by MDPI (http://www.mdpi.org). Reproduction is permitted for noncommercial purposes.
spellingShingle Full Research Paper
De Stefano, Luca
Arcari, Paolo
Lamberti, Annalisa
Sanges, Carmen
Rotiroti, Lucia
Rea, Ilaria
Rendina, Ivo
DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips
title DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips
title_full DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips
title_fullStr DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips
title_full_unstemmed DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips
title_short DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips
title_sort dna optical detection based on porous silicon technology: from biosensors to biochips
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756702/
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