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A Hydrogen Peroxide Sensor Prepared by Electropolymerization of Pyrrole Based on Screen-Printed Carbon Paste Electrodes

A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enz...

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Detalles Bibliográficos
Autores principales: Li, Guang, Wang, You, Xu, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756719/
Descripción
Sumario:A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enzyme electrodes are suitable for mass production and quality control. The biosensor shows a linear amperometric response to H(2)O(2) from 0.1 to 2.0 mM, with a sensitivity of 33.24 µA mM(-1) cm(-2). Different operational parameters of electropolymerization are evaluated and optimized.