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Anticoagulant effect of Huisheng oral solution in a rat model of thrombosis

OBJECTIVE: To investigate whether Huisheng Oral Solution has an anticoagulant effect in a rat model of thrombosis. MATERIALS AND METHODS: A total of 40 male SD rats were equally and randomly divided into four groups: blank group, model group, and two treatment groups (A and B). Rats were subcutaneou...

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Autores principales: Liu, Shuang-Qing, Guo, Jian-Ying, Du, Jun, Deng, Qun, He, Zhong-Jie, Lin, Hong-Yuan, Lei, Shu-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757604/
https://www.ncbi.nlm.nih.gov/pubmed/24014911
http://dx.doi.org/10.4103/0253-7613.115018
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author Liu, Shuang-Qing
Guo, Jian-Ying
Du, Jun
Deng, Qun
He, Zhong-Jie
Lin, Hong-Yuan
Lei, Shu-Hong
author_facet Liu, Shuang-Qing
Guo, Jian-Ying
Du, Jun
Deng, Qun
He, Zhong-Jie
Lin, Hong-Yuan
Lei, Shu-Hong
author_sort Liu, Shuang-Qing
collection PubMed
description OBJECTIVE: To investigate whether Huisheng Oral Solution has an anticoagulant effect in a rat model of thrombosis. MATERIALS AND METHODS: A total of 40 male SD rats were equally and randomly divided into four groups: blank group, model group, and two treatment groups (A and B). Rats were subcutaneously injected with carrageenan to induce thrombosis. Rats in the treatment group A were intragastrically administered with Huisheng Oral Solution at a dose of 2 ml/100 g body weight (once per 8 hours), 72 hours after carrageenan injection, while those in the treatment group B were administered with Huisheng Oral Solution both 72 hours before and after induction of thrombosis. Blood samples were collected 24, 48, and 72 hours after carrageenan injection for measurements of prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio (INR), fibrinogen (FIB), prothrombin activity (PTA), platelets (PLT), fibrin degradation products (FDPs), and D-dimer. Lung, liver, and mesentery samples were taken 72 hours after carrageenan injection for histopathological analysis. The numbers of microthrombi in sections of different tissue samples were counted under a microscope. Blood parameters among each group were compared using the Welch test, the Kruskal-Wallis test, or the SNK test after testing for normality, while the number of microthrombi was compared using the Bonferroni test. RESULTS: Compared to those in the model group, PT, APTT, and INR were significantly prolonged or increased while FIB was significantly reduced at the majority of time points in the two treatment groups (P < 0.05 for all). The levels of FDPs and D-dimer and PLT counts at the majority of time points were significantly lower (P < 0.05 for all), and the numbers of microthrombi in lung, liver, and mesentery samples were significantly decreased (P < 0.05 for all) in the two treatment groups. The above parameters at the majority of time points showed no significant differences between the two treatment groups. CONCLUSIONS: Huisheng Oral Solution can significantly improve coagulation parameters, fibrinolysis parameters, and PLT count, and reduce blood hypercoagulability and microthrombosis, suggesting that Huisheng Oral Solution has an anticoagulant effect in a rat model of thrombosis.
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spelling pubmed-37576042013-09-06 Anticoagulant effect of Huisheng oral solution in a rat model of thrombosis Liu, Shuang-Qing Guo, Jian-Ying Du, Jun Deng, Qun He, Zhong-Jie Lin, Hong-Yuan Lei, Shu-Hong Indian J Pharmacol Research Article OBJECTIVE: To investigate whether Huisheng Oral Solution has an anticoagulant effect in a rat model of thrombosis. MATERIALS AND METHODS: A total of 40 male SD rats were equally and randomly divided into four groups: blank group, model group, and two treatment groups (A and B). Rats were subcutaneously injected with carrageenan to induce thrombosis. Rats in the treatment group A were intragastrically administered with Huisheng Oral Solution at a dose of 2 ml/100 g body weight (once per 8 hours), 72 hours after carrageenan injection, while those in the treatment group B were administered with Huisheng Oral Solution both 72 hours before and after induction of thrombosis. Blood samples were collected 24, 48, and 72 hours after carrageenan injection for measurements of prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio (INR), fibrinogen (FIB), prothrombin activity (PTA), platelets (PLT), fibrin degradation products (FDPs), and D-dimer. Lung, liver, and mesentery samples were taken 72 hours after carrageenan injection for histopathological analysis. The numbers of microthrombi in sections of different tissue samples were counted under a microscope. Blood parameters among each group were compared using the Welch test, the Kruskal-Wallis test, or the SNK test after testing for normality, while the number of microthrombi was compared using the Bonferroni test. RESULTS: Compared to those in the model group, PT, APTT, and INR were significantly prolonged or increased while FIB was significantly reduced at the majority of time points in the two treatment groups (P < 0.05 for all). The levels of FDPs and D-dimer and PLT counts at the majority of time points were significantly lower (P < 0.05 for all), and the numbers of microthrombi in lung, liver, and mesentery samples were significantly decreased (P < 0.05 for all) in the two treatment groups. The above parameters at the majority of time points showed no significant differences between the two treatment groups. CONCLUSIONS: Huisheng Oral Solution can significantly improve coagulation parameters, fibrinolysis parameters, and PLT count, and reduce blood hypercoagulability and microthrombosis, suggesting that Huisheng Oral Solution has an anticoagulant effect in a rat model of thrombosis. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3757604/ /pubmed/24014911 http://dx.doi.org/10.4103/0253-7613.115018 Text en Copyright: © Indian Journal of Pharmacology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Shuang-Qing
Guo, Jian-Ying
Du, Jun
Deng, Qun
He, Zhong-Jie
Lin, Hong-Yuan
Lei, Shu-Hong
Anticoagulant effect of Huisheng oral solution in a rat model of thrombosis
title Anticoagulant effect of Huisheng oral solution in a rat model of thrombosis
title_full Anticoagulant effect of Huisheng oral solution in a rat model of thrombosis
title_fullStr Anticoagulant effect of Huisheng oral solution in a rat model of thrombosis
title_full_unstemmed Anticoagulant effect of Huisheng oral solution in a rat model of thrombosis
title_short Anticoagulant effect of Huisheng oral solution in a rat model of thrombosis
title_sort anticoagulant effect of huisheng oral solution in a rat model of thrombosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757604/
https://www.ncbi.nlm.nih.gov/pubmed/24014911
http://dx.doi.org/10.4103/0253-7613.115018
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