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Protective effect of Clerodendrum colebrookianum Walp., on acute and chronic inflammation in rats
AIM: To evaluate antioxidant, anti-inflammatory potential of the aqueous extracts and its aqueous, n-butanol, ethyl-acetate, and chloroform fractions of Clerodendrum colebrookianum Walp. leaves. MATERIALS AND METHODS: In this present study, all the test samples were evaluated on in-vivo inflammatory...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757607/ https://www.ncbi.nlm.nih.gov/pubmed/24014914 http://dx.doi.org/10.4103/0253-7613.115021 |
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author | Deb, Lokesh Dey, Amitabha Sakthivel, G. Bhattamishra, Subrat Kumar Dutta, Amitsankar |
author_facet | Deb, Lokesh Dey, Amitabha Sakthivel, G. Bhattamishra, Subrat Kumar Dutta, Amitsankar |
author_sort | Deb, Lokesh |
collection | PubMed |
description | AIM: To evaluate antioxidant, anti-inflammatory potential of the aqueous extracts and its aqueous, n-butanol, ethyl-acetate, and chloroform fractions of Clerodendrum colebrookianum Walp. leaves. MATERIALS AND METHODS: In this present study, all the test samples were evaluated on in-vivo inflammatory model such as carrageenan and histamine-induced acute-inflammation and cotton pellet induced granuloma formation in albino male rats. Test samples were also employed in in-vitro assays like DPPH* free radical scavenging activity and COX inhibition assay. RESULTS: The test samples at the dose of 200mg/kg/p.o. were found to cause significant inhibition of carrageenan and histamine-induced inflammation and cotton pallet-induced granuloma formation on acute and chronic inflammation in rats. The test samples, except n-butanol fraction, exhibited inhibitory effect for both COX-1 and COX-2, in in-vitro assay but their percentage of inhibition values differs from each other. The test samples (aqueous extracts, aqueous, n-butanol, ethyl-acetate, and chloroform fractions) at 100 μg concentration exhibits 54.37%, 33.88%, 62.85%, 56.28%, and 57.48% DPPH* radical-scavenging effect respectively in in-vitro antioxidant study. CONCLUSION: These observations established the anti-inflammatory effect of C. colebrookianum leaves in acute and chronic stages of inflammation by free radical scavenging and inhibition of COX-1 and COX-2. |
format | Online Article Text |
id | pubmed-3757607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-37576072013-09-06 Protective effect of Clerodendrum colebrookianum Walp., on acute and chronic inflammation in rats Deb, Lokesh Dey, Amitabha Sakthivel, G. Bhattamishra, Subrat Kumar Dutta, Amitsankar Indian J Pharmacol Research Article AIM: To evaluate antioxidant, anti-inflammatory potential of the aqueous extracts and its aqueous, n-butanol, ethyl-acetate, and chloroform fractions of Clerodendrum colebrookianum Walp. leaves. MATERIALS AND METHODS: In this present study, all the test samples were evaluated on in-vivo inflammatory model such as carrageenan and histamine-induced acute-inflammation and cotton pellet induced granuloma formation in albino male rats. Test samples were also employed in in-vitro assays like DPPH* free radical scavenging activity and COX inhibition assay. RESULTS: The test samples at the dose of 200mg/kg/p.o. were found to cause significant inhibition of carrageenan and histamine-induced inflammation and cotton pallet-induced granuloma formation on acute and chronic inflammation in rats. The test samples, except n-butanol fraction, exhibited inhibitory effect for both COX-1 and COX-2, in in-vitro assay but their percentage of inhibition values differs from each other. The test samples (aqueous extracts, aqueous, n-butanol, ethyl-acetate, and chloroform fractions) at 100 μg concentration exhibits 54.37%, 33.88%, 62.85%, 56.28%, and 57.48% DPPH* radical-scavenging effect respectively in in-vitro antioxidant study. CONCLUSION: These observations established the anti-inflammatory effect of C. colebrookianum leaves in acute and chronic stages of inflammation by free radical scavenging and inhibition of COX-1 and COX-2. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3757607/ /pubmed/24014914 http://dx.doi.org/10.4103/0253-7613.115021 Text en Copyright: © Indian Journal of Pharmacology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Deb, Lokesh Dey, Amitabha Sakthivel, G. Bhattamishra, Subrat Kumar Dutta, Amitsankar Protective effect of Clerodendrum colebrookianum Walp., on acute and chronic inflammation in rats |
title | Protective effect of Clerodendrum colebrookianum Walp., on acute and chronic inflammation in rats |
title_full | Protective effect of Clerodendrum colebrookianum Walp., on acute and chronic inflammation in rats |
title_fullStr | Protective effect of Clerodendrum colebrookianum Walp., on acute and chronic inflammation in rats |
title_full_unstemmed | Protective effect of Clerodendrum colebrookianum Walp., on acute and chronic inflammation in rats |
title_short | Protective effect of Clerodendrum colebrookianum Walp., on acute and chronic inflammation in rats |
title_sort | protective effect of clerodendrum colebrookianum walp., on acute and chronic inflammation in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757607/ https://www.ncbi.nlm.nih.gov/pubmed/24014914 http://dx.doi.org/10.4103/0253-7613.115021 |
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