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Characterizing low dose and dose rate effects in rodent and human neural stem cells exposed to proton and gamma irradiation()

Past work has shown that exposure to gamma rays and protons elicit a persistent oxidative stress in rodent and human neural stem cells (hNSCs). We have now adapted these studies to more realistic exposure scenarios in space, using lower doses and dose rates of these radiation modalities, to further...

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Autores principales: Tseng, Bertrand P., Lan, Mary L., Tran, Katherine K., Acharya, Munjal M., Giedzinski, Erich, Limoli, Charles L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757683/
https://www.ncbi.nlm.nih.gov/pubmed/24024148
http://dx.doi.org/10.1016/j.redox.2013.01.008
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author Tseng, Bertrand P.
Lan, Mary L.
Tran, Katherine K.
Acharya, Munjal M.
Giedzinski, Erich
Limoli, Charles L.
author_facet Tseng, Bertrand P.
Lan, Mary L.
Tran, Katherine K.
Acharya, Munjal M.
Giedzinski, Erich
Limoli, Charles L.
author_sort Tseng, Bertrand P.
collection PubMed
description Past work has shown that exposure to gamma rays and protons elicit a persistent oxidative stress in rodent and human neural stem cells (hNSCs). We have now adapted these studies to more realistic exposure scenarios in space, using lower doses and dose rates of these radiation modalities, to further elucidate the role of radiation-induced oxidative stress in these cells. Rodent neural stem and precursor cells grown as neurospheres and human neural stem cells grown as monolayers were subjected to acute and multi-dosing paradigms at differing dose rates and analyzed for changes in reactive oxygen species (ROS), reactive nitrogen species (RNS), nitric oxide and superoxide for 2 days after irradiation. While acute exposures led to significant changes in both cell types, hNSCs in particular, exhibited marked and significant elevations in radiation-induced oxidative stress. Elevated oxidative stress was more significant in hNSCs as opposed to their rodent counterparts, and hNSCs were significantly more sensitive to low dose exposures in terms of survival. Combinations of protons and γ-rays delivered as lower priming or higher challenge doses elicited radioadaptive changes that were associated with improved survival, but in general, only under conditions where the levels of reactive species were suppressed compared to cells irradiated acutely. Protective radioadaptive effects on survival were eliminated in the presence of the antioxidant N-acetylcysteine, suggesting further that radiation-induced oxidative stress could activate pro-survival signaling pathways that were sensitive to redox state. Data corroborates much of our past work and shows that low dose and dose rate exposures elicit significant changes in oxidative stress that have functional consequences on survival.
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spelling pubmed-37576832013-09-10 Characterizing low dose and dose rate effects in rodent and human neural stem cells exposed to proton and gamma irradiation() Tseng, Bertrand P. Lan, Mary L. Tran, Katherine K. Acharya, Munjal M. Giedzinski, Erich Limoli, Charles L. Redox Biol Research Paper Past work has shown that exposure to gamma rays and protons elicit a persistent oxidative stress in rodent and human neural stem cells (hNSCs). We have now adapted these studies to more realistic exposure scenarios in space, using lower doses and dose rates of these radiation modalities, to further elucidate the role of radiation-induced oxidative stress in these cells. Rodent neural stem and precursor cells grown as neurospheres and human neural stem cells grown as monolayers were subjected to acute and multi-dosing paradigms at differing dose rates and analyzed for changes in reactive oxygen species (ROS), reactive nitrogen species (RNS), nitric oxide and superoxide for 2 days after irradiation. While acute exposures led to significant changes in both cell types, hNSCs in particular, exhibited marked and significant elevations in radiation-induced oxidative stress. Elevated oxidative stress was more significant in hNSCs as opposed to their rodent counterparts, and hNSCs were significantly more sensitive to low dose exposures in terms of survival. Combinations of protons and γ-rays delivered as lower priming or higher challenge doses elicited radioadaptive changes that were associated with improved survival, but in general, only under conditions where the levels of reactive species were suppressed compared to cells irradiated acutely. Protective radioadaptive effects on survival were eliminated in the presence of the antioxidant N-acetylcysteine, suggesting further that radiation-induced oxidative stress could activate pro-survival signaling pathways that were sensitive to redox state. Data corroborates much of our past work and shows that low dose and dose rate exposures elicit significant changes in oxidative stress that have functional consequences on survival. Elsevier 2013-01-19 /pmc/articles/PMC3757683/ /pubmed/24024148 http://dx.doi.org/10.1016/j.redox.2013.01.008 Text en © 2013 The Authors http://creativecommons.org/licenses/BY-license/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Tseng, Bertrand P.
Lan, Mary L.
Tran, Katherine K.
Acharya, Munjal M.
Giedzinski, Erich
Limoli, Charles L.
Characterizing low dose and dose rate effects in rodent and human neural stem cells exposed to proton and gamma irradiation()
title Characterizing low dose and dose rate effects in rodent and human neural stem cells exposed to proton and gamma irradiation()
title_full Characterizing low dose and dose rate effects in rodent and human neural stem cells exposed to proton and gamma irradiation()
title_fullStr Characterizing low dose and dose rate effects in rodent and human neural stem cells exposed to proton and gamma irradiation()
title_full_unstemmed Characterizing low dose and dose rate effects in rodent and human neural stem cells exposed to proton and gamma irradiation()
title_short Characterizing low dose and dose rate effects in rodent and human neural stem cells exposed to proton and gamma irradiation()
title_sort characterizing low dose and dose rate effects in rodent and human neural stem cells exposed to proton and gamma irradiation()
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757683/
https://www.ncbi.nlm.nih.gov/pubmed/24024148
http://dx.doi.org/10.1016/j.redox.2013.01.008
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