Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain

Neural stem/progenitor cells (NSPCs) generate new neurons throughout life in distinct areas of the adult mammalian brain. Besides classical transgenesis-based approaches, retrovirus-mediated genetic manipulation is frequently used to study mechanisms that regulate neurogenesis in the nervous system....

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Detalles Bibliográficos
Autores principales: Braun, Simon M.G., Machado, Raquel A.C., Jessberger, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757750/
https://www.ncbi.nlm.nih.gov/pubmed/24052947
http://dx.doi.org/10.1016/j.stemcr.2013.06.003
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author Braun, Simon M.G.
Machado, Raquel A.C.
Jessberger, Sebastian
author_facet Braun, Simon M.G.
Machado, Raquel A.C.
Jessberger, Sebastian
author_sort Braun, Simon M.G.
collection PubMed
description Neural stem/progenitor cells (NSPCs) generate new neurons throughout life in distinct areas of the adult mammalian brain. Besides classical transgenesis-based approaches, retrovirus-mediated genetic manipulation is frequently used to study mechanisms that regulate neurogenesis in the nervous system. Here, we show that fusion of a tamoxifen-regulatable estrogen receptor (ER(T2)) motif to transcription factors (i.e., ASCL1 and NEUROD1) enables temporal control of transgene expression in adult mouse NSPCs in vitro and in vivo. Thus, the approach described here represents a versatile strategy for regulating gene expression to study gene function in dividing cells and their progeny.
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spelling pubmed-37577502013-09-17 Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain Braun, Simon M.G. Machado, Raquel A.C. Jessberger, Sebastian Stem Cell Reports Report Neural stem/progenitor cells (NSPCs) generate new neurons throughout life in distinct areas of the adult mammalian brain. Besides classical transgenesis-based approaches, retrovirus-mediated genetic manipulation is frequently used to study mechanisms that regulate neurogenesis in the nervous system. Here, we show that fusion of a tamoxifen-regulatable estrogen receptor (ER(T2)) motif to transcription factors (i.e., ASCL1 and NEUROD1) enables temporal control of transgene expression in adult mouse NSPCs in vitro and in vivo. Thus, the approach described here represents a versatile strategy for regulating gene expression to study gene function in dividing cells and their progeny. Elsevier 2013-07-11 /pmc/articles/PMC3757750/ /pubmed/24052947 http://dx.doi.org/10.1016/j.stemcr.2013.06.003 Text en © 2013 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Report
Braun, Simon M.G.
Machado, Raquel A.C.
Jessberger, Sebastian
Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain
title Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain
title_full Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain
title_fullStr Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain
title_full_unstemmed Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain
title_short Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain
title_sort temporal control of retroviral transgene expression in newborn cells in the adult brain
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757750/
https://www.ncbi.nlm.nih.gov/pubmed/24052947
http://dx.doi.org/10.1016/j.stemcr.2013.06.003
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