Do Pluripotent Stem Cells Exist in Adult Mice as Very Small Embryonic Stem Cells?

Very small embryonic-like stem cells (VSELs) isolated from bone marrow (BM) have been reported to be pluripotent. Given their nonembryonic source, they could replace blastocyst-derived embryonic stem cells in research and medicine. However, their multiple-germ-layer potential has been incompletely s...

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Autores principales: Miyanishi, Masanori, Mori, Yasuo, Seita, Jun, Chen, James Y., Karten, Seth, Chan, Charles K.F., Nakauchi, Hiromitsu, Weissman, Irving L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757755/
https://www.ncbi.nlm.nih.gov/pubmed/24052953
http://dx.doi.org/10.1016/j.stemcr.2013.07.001
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author Miyanishi, Masanori
Mori, Yasuo
Seita, Jun
Chen, James Y.
Karten, Seth
Chan, Charles K.F.
Nakauchi, Hiromitsu
Weissman, Irving L.
author_facet Miyanishi, Masanori
Mori, Yasuo
Seita, Jun
Chen, James Y.
Karten, Seth
Chan, Charles K.F.
Nakauchi, Hiromitsu
Weissman, Irving L.
author_sort Miyanishi, Masanori
collection PubMed
description Very small embryonic-like stem cells (VSELs) isolated from bone marrow (BM) have been reported to be pluripotent. Given their nonembryonic source, they could replace blastocyst-derived embryonic stem cells in research and medicine. However, their multiple-germ-layer potential has been incompletely studied. Here, we show that we cannot find VSELs in mouse BM with any of the reported stem cell potentials, specifically for hematopoiesis. We found that: (1) most events within the “VSEL” flow-cytometry gate had little DNA and the cells corresponding to these events (2) could not form spheres, (3) did not express Oct4, and (4) could not differentiate into blood cells. These results provide a failure to confirm the existence of pluripotent VSELs.
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spelling pubmed-37577552013-09-17 Do Pluripotent Stem Cells Exist in Adult Mice as Very Small Embryonic Stem Cells? Miyanishi, Masanori Mori, Yasuo Seita, Jun Chen, James Y. Karten, Seth Chan, Charles K.F. Nakauchi, Hiromitsu Weissman, Irving L. Stem Cell Reports Article Very small embryonic-like stem cells (VSELs) isolated from bone marrow (BM) have been reported to be pluripotent. Given their nonembryonic source, they could replace blastocyst-derived embryonic stem cells in research and medicine. However, their multiple-germ-layer potential has been incompletely studied. Here, we show that we cannot find VSELs in mouse BM with any of the reported stem cell potentials, specifically for hematopoiesis. We found that: (1) most events within the “VSEL” flow-cytometry gate had little DNA and the cells corresponding to these events (2) could not form spheres, (3) did not express Oct4, and (4) could not differentiate into blood cells. These results provide a failure to confirm the existence of pluripotent VSELs. Elsevier 2013-07-24 /pmc/articles/PMC3757755/ /pubmed/24052953 http://dx.doi.org/10.1016/j.stemcr.2013.07.001 Text en © 2013 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Miyanishi, Masanori
Mori, Yasuo
Seita, Jun
Chen, James Y.
Karten, Seth
Chan, Charles K.F.
Nakauchi, Hiromitsu
Weissman, Irving L.
Do Pluripotent Stem Cells Exist in Adult Mice as Very Small Embryonic Stem Cells?
title Do Pluripotent Stem Cells Exist in Adult Mice as Very Small Embryonic Stem Cells?
title_full Do Pluripotent Stem Cells Exist in Adult Mice as Very Small Embryonic Stem Cells?
title_fullStr Do Pluripotent Stem Cells Exist in Adult Mice as Very Small Embryonic Stem Cells?
title_full_unstemmed Do Pluripotent Stem Cells Exist in Adult Mice as Very Small Embryonic Stem Cells?
title_short Do Pluripotent Stem Cells Exist in Adult Mice as Very Small Embryonic Stem Cells?
title_sort do pluripotent stem cells exist in adult mice as very small embryonic stem cells?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757755/
https://www.ncbi.nlm.nih.gov/pubmed/24052953
http://dx.doi.org/10.1016/j.stemcr.2013.07.001
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