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Purification, crystallization and preliminary X-ray diffraction analysis of the 23S rRNA methyltransferase RlmJ from Escherichia coli

Methyltransferase RlmJ uses the cofactor S-adenosylmethionine to methylate the exocyclic nitrogen N6 of nucleotide A2030 in 23S rRNA during ribosome assembly in Escherichia coli. RlmJ with a C-terminal hexahistidine tag was overexpressed in E. coli and purified as a monomer using Ni(2+)-affinity and...

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Detalles Bibliográficos
Autores principales:	Punekar, Avinash S., Selmer, Maria
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	International Union of Crystallography 2013
Materias:	Crystallization Communications
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3758148/ https://www.ncbi.nlm.nih.gov/pubmed/23989148 http://dx.doi.org/10.1107/S1744309113020289

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