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Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold
BACKGROUND: Peripheral nerve injury is a common trauma, but presents a significant challenge to the clinic. Silk-based materials have recently become an important biomaterial for tissue engineering applications due to silk’s biocompatibility and impressive mechanical and degradative properties. In t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3758218/ https://www.ncbi.nlm.nih.gov/pubmed/24009419 http://dx.doi.org/10.2147/IJN.S43681 |
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author | Wei, Guo-Jun Yao, Meng Wang, Yan-Song Zhou, Chang-Wei Wan, De-Yu Lei, Peng-Zhen Wen, Jian Lei, Hong-Wei Dong, Da-Ming |
author_facet | Wei, Guo-Jun Yao, Meng Wang, Yan-Song Zhou, Chang-Wei Wan, De-Yu Lei, Peng-Zhen Wen, Jian Lei, Hong-Wei Dong, Da-Ming |
author_sort | Wei, Guo-Jun |
collection | PubMed |
description | BACKGROUND: Peripheral nerve injury is a common trauma, but presents a significant challenge to the clinic. Silk-based materials have recently become an important biomaterial for tissue engineering applications due to silk’s biocompatibility and impressive mechanical and degradative properties. In the present study, a silk fibroin peptide (SF16) was designed and used as a component of the hydrogel scaffold for the repair of peripheral nerve injury. METHODS: The SF16 peptide’s structure was characterized using spectrophotometry and atomic force microscopy, and the SF16 hydrogel was analyzed using scanning electron microscopy. The effects of the SF16 hydrogel on the viability and growth of live cells was first assessed in vitro, on PC12 cells. The in vivo test model involved the repair of a nerve gap with tubular nerve guides, through which it was possible to identify if the SF16 hydrogel would have the potential to enhance nerve regeneration. In this model physiological saline was set as the negative control, and collagen as the positive control. Walking track analysis and electrophysiological methods were used to evaluate the functional recovery of the nerve at 4 and 8 weeks after surgery. RESULTS: Analysis of the SF16 peptide’s characteristics indicated that it consisted of a well-defined secondary structure and exhibited self-assembly. Results of scanning electron microscopy showed that the peptide based hydrogel may represent a porous scaffold that is viable for repair of peripheral nerve injury. Analysis of cell culture also supported that the hydrogel was an effective matrix to maintain the viability, morphology and proliferation of PC12 cells. Electrophysiology demonstrated that the use of the hydrogel scaffold (SF16 or collagen) resulted in a significant improvement in amplitude recovery in the in vivo model compared to physiological saline. Moreover, nerve cells in the SF16 hydrogel group displayed greater axon density, larger average axon diameter and thicker myelin compared to those of the group that received physiological saline. CONCLUSION: The SF16 hydrogel scaffold may promote excellent axonal regeneration and functional recovery after peripheral nerve injury, and the SF16 peptide may be a candidate for nerve tissue engineering applications. |
format | Online Article Text |
id | pubmed-3758218 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-37582182013-09-05 Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold Wei, Guo-Jun Yao, Meng Wang, Yan-Song Zhou, Chang-Wei Wan, De-Yu Lei, Peng-Zhen Wen, Jian Lei, Hong-Wei Dong, Da-Ming Int J Nanomedicine Original Research BACKGROUND: Peripheral nerve injury is a common trauma, but presents a significant challenge to the clinic. Silk-based materials have recently become an important biomaterial for tissue engineering applications due to silk’s biocompatibility and impressive mechanical and degradative properties. In the present study, a silk fibroin peptide (SF16) was designed and used as a component of the hydrogel scaffold for the repair of peripheral nerve injury. METHODS: The SF16 peptide’s structure was characterized using spectrophotometry and atomic force microscopy, and the SF16 hydrogel was analyzed using scanning electron microscopy. The effects of the SF16 hydrogel on the viability and growth of live cells was first assessed in vitro, on PC12 cells. The in vivo test model involved the repair of a nerve gap with tubular nerve guides, through which it was possible to identify if the SF16 hydrogel would have the potential to enhance nerve regeneration. In this model physiological saline was set as the negative control, and collagen as the positive control. Walking track analysis and electrophysiological methods were used to evaluate the functional recovery of the nerve at 4 and 8 weeks after surgery. RESULTS: Analysis of the SF16 peptide’s characteristics indicated that it consisted of a well-defined secondary structure and exhibited self-assembly. Results of scanning electron microscopy showed that the peptide based hydrogel may represent a porous scaffold that is viable for repair of peripheral nerve injury. Analysis of cell culture also supported that the hydrogel was an effective matrix to maintain the viability, morphology and proliferation of PC12 cells. Electrophysiology demonstrated that the use of the hydrogel scaffold (SF16 or collagen) resulted in a significant improvement in amplitude recovery in the in vivo model compared to physiological saline. Moreover, nerve cells in the SF16 hydrogel group displayed greater axon density, larger average axon diameter and thicker myelin compared to those of the group that received physiological saline. CONCLUSION: The SF16 hydrogel scaffold may promote excellent axonal regeneration and functional recovery after peripheral nerve injury, and the SF16 peptide may be a candidate for nerve tissue engineering applications. Dove Medical Press 2013 2013-08-22 /pmc/articles/PMC3758218/ /pubmed/24009419 http://dx.doi.org/10.2147/IJN.S43681 Text en © 2013 Wei et al, publisher and licensee Dove Medical Press Ltd This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Wei, Guo-Jun Yao, Meng Wang, Yan-Song Zhou, Chang-Wei Wan, De-Yu Lei, Peng-Zhen Wen, Jian Lei, Hong-Wei Dong, Da-Ming Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold |
title | Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold |
title_full | Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold |
title_fullStr | Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold |
title_full_unstemmed | Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold |
title_short | Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold |
title_sort | promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3758218/ https://www.ncbi.nlm.nih.gov/pubmed/24009419 http://dx.doi.org/10.2147/IJN.S43681 |
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