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Characterization of Treatment-Naive HIV/HBV Co-Infected Patients Attending ART Clinic of a Tertiary Healthcare Centre in Eastern India

OBJECTIVE: The study was designed to assess the hepatitis B virus (HBV) and hepatitis C virus (HCV) co-infection scenario among the human immunodeficiency virus (HIV) infected patients attending a tertiary healthcare unit in eastern India. Additionally, clinical and virological characterization of t...

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Autores principales: Saha, Debraj, Pal, Ananya, Biswas, Avik, Panigrahi, Rajesh, Sarkar, Neelakshi, Sarkar, Jayeeta, Pal, Manisha, Guha, Subhasish Kamal, Saha, Bibhuti, Chakrabarti, Sekhar, Chakravarty, Runu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3758335/
https://www.ncbi.nlm.nih.gov/pubmed/24023688
http://dx.doi.org/10.1371/journal.pone.0073613
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author Saha, Debraj
Pal, Ananya
Biswas, Avik
Panigrahi, Rajesh
Sarkar, Neelakshi
Sarkar, Jayeeta
Pal, Manisha
Guha, Subhasish Kamal
Saha, Bibhuti
Chakrabarti, Sekhar
Chakravarty, Runu
author_facet Saha, Debraj
Pal, Ananya
Biswas, Avik
Panigrahi, Rajesh
Sarkar, Neelakshi
Sarkar, Jayeeta
Pal, Manisha
Guha, Subhasish Kamal
Saha, Bibhuti
Chakrabarti, Sekhar
Chakravarty, Runu
author_sort Saha, Debraj
collection PubMed
description OBJECTIVE: The study was designed to assess the hepatitis B virus (HBV) and hepatitis C virus (HCV) co-infection scenario among the human immunodeficiency virus (HIV) infected patients attending a tertiary healthcare unit in eastern India. Additionally, clinical and virological characterization of these viruses, prior to antiretroviral therapy (ART) initiation was also done for better understanding of the disease profile. METHODS: Pool of ART-naive HIV/HBV co-infected and HIV mono-infected patients, participating in two different studies, were included in this study. HBV DNA was detected by nested-PCR amplification followed by HBV genotype determination and HBV reverse transcriptase (RT) region amplification and direct sequencing for detecting drug resistance. RESULTS: The prevalence of HBsAg (11.3%) was higher compared to anti-HCV (1.9%) among the HIV infected ART-naive patients. Moreover, majority of the HBeAg positive HIV/HBV co-infected patients (87.7%) had HBV DNA ≥20,000 IU/ml with median HBV DNA significantly higher than that of HBeAg negative subjects (5.7 log(10) IU/ml vs. 4.2 log(10) IU/ml; p<0.0001). Multivariate analysis also showed that HBeAg-positive status was independently associated with higher HBV DNA level (p = <0.001). Notably, 60.9% of the HBeAg negative co-infected subjects had HBV DNA ≥2,000 IU/ml of which 37.0% had HBV DNA ≥20,000 IU/ml. Genotype HBV/D (68.2%) was the predominant genotype followed by HBV/A (24.3%) and HBV/C (7.5%). Anti-HBV drug resistant mutations were detected in two (3.8%) of the ART-naive patients. CONCLUSION: The prevalence of HIV/HBV co-infection was relatively higher in our study subjects. HBeAg testing might provide clue for early treatment initiation. Furthermore, HBeAg negative patients are also associated with high HBV DNA levels and therefore require appropriate medical attention. Pre-treatment screening for anti-HBV drug resistant mutations is not necessary before ART initiation.
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spelling pubmed-37583352013-09-10 Characterization of Treatment-Naive HIV/HBV Co-Infected Patients Attending ART Clinic of a Tertiary Healthcare Centre in Eastern India Saha, Debraj Pal, Ananya Biswas, Avik Panigrahi, Rajesh Sarkar, Neelakshi Sarkar, Jayeeta Pal, Manisha Guha, Subhasish Kamal Saha, Bibhuti Chakrabarti, Sekhar Chakravarty, Runu PLoS One Research Article OBJECTIVE: The study was designed to assess the hepatitis B virus (HBV) and hepatitis C virus (HCV) co-infection scenario among the human immunodeficiency virus (HIV) infected patients attending a tertiary healthcare unit in eastern India. Additionally, clinical and virological characterization of these viruses, prior to antiretroviral therapy (ART) initiation was also done for better understanding of the disease profile. METHODS: Pool of ART-naive HIV/HBV co-infected and HIV mono-infected patients, participating in two different studies, were included in this study. HBV DNA was detected by nested-PCR amplification followed by HBV genotype determination and HBV reverse transcriptase (RT) region amplification and direct sequencing for detecting drug resistance. RESULTS: The prevalence of HBsAg (11.3%) was higher compared to anti-HCV (1.9%) among the HIV infected ART-naive patients. Moreover, majority of the HBeAg positive HIV/HBV co-infected patients (87.7%) had HBV DNA ≥20,000 IU/ml with median HBV DNA significantly higher than that of HBeAg negative subjects (5.7 log(10) IU/ml vs. 4.2 log(10) IU/ml; p<0.0001). Multivariate analysis also showed that HBeAg-positive status was independently associated with higher HBV DNA level (p = <0.001). Notably, 60.9% of the HBeAg negative co-infected subjects had HBV DNA ≥2,000 IU/ml of which 37.0% had HBV DNA ≥20,000 IU/ml. Genotype HBV/D (68.2%) was the predominant genotype followed by HBV/A (24.3%) and HBV/C (7.5%). Anti-HBV drug resistant mutations were detected in two (3.8%) of the ART-naive patients. CONCLUSION: The prevalence of HIV/HBV co-infection was relatively higher in our study subjects. HBeAg testing might provide clue for early treatment initiation. Furthermore, HBeAg negative patients are also associated with high HBV DNA levels and therefore require appropriate medical attention. Pre-treatment screening for anti-HBV drug resistant mutations is not necessary before ART initiation. Public Library of Science 2013-08-30 /pmc/articles/PMC3758335/ /pubmed/24023688 http://dx.doi.org/10.1371/journal.pone.0073613 Text en © 2013 Saha et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Saha, Debraj
Pal, Ananya
Biswas, Avik
Panigrahi, Rajesh
Sarkar, Neelakshi
Sarkar, Jayeeta
Pal, Manisha
Guha, Subhasish Kamal
Saha, Bibhuti
Chakrabarti, Sekhar
Chakravarty, Runu
Characterization of Treatment-Naive HIV/HBV Co-Infected Patients Attending ART Clinic of a Tertiary Healthcare Centre in Eastern India
title Characterization of Treatment-Naive HIV/HBV Co-Infected Patients Attending ART Clinic of a Tertiary Healthcare Centre in Eastern India
title_full Characterization of Treatment-Naive HIV/HBV Co-Infected Patients Attending ART Clinic of a Tertiary Healthcare Centre in Eastern India
title_fullStr Characterization of Treatment-Naive HIV/HBV Co-Infected Patients Attending ART Clinic of a Tertiary Healthcare Centre in Eastern India
title_full_unstemmed Characterization of Treatment-Naive HIV/HBV Co-Infected Patients Attending ART Clinic of a Tertiary Healthcare Centre in Eastern India
title_short Characterization of Treatment-Naive HIV/HBV Co-Infected Patients Attending ART Clinic of a Tertiary Healthcare Centre in Eastern India
title_sort characterization of treatment-naive hiv/hbv co-infected patients attending art clinic of a tertiary healthcare centre in eastern india
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3758335/
https://www.ncbi.nlm.nih.gov/pubmed/24023688
http://dx.doi.org/10.1371/journal.pone.0073613
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