Imaging bacterial protein expression using genetically encoded sensors composed of RNA

We show that the difficulties in imaging the dynamics of protein expression in live bacterial cells can be overcome using fluorescent sensors based on Spinach, an RNA that activates the fluorescence of a small-molecule fluorophore. These RNAs selectively bind target proteins, and exhibit fluorescenc...

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Detalles Bibliográficos
Autores principales: Song, Wenjiao, Strack, Rita L., Jaffrey, Samie R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3758421/
https://www.ncbi.nlm.nih.gov/pubmed/23872791
http://dx.doi.org/10.1038/nmeth.2568
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author Song, Wenjiao
Strack, Rita L.
Jaffrey, Samie R.
author_facet Song, Wenjiao
Strack, Rita L.
Jaffrey, Samie R.
author_sort Song, Wenjiao
collection PubMed
description We show that the difficulties in imaging the dynamics of protein expression in live bacterial cells can be overcome using fluorescent sensors based on Spinach, an RNA that activates the fluorescence of a small-molecule fluorophore. These RNAs selectively bind target proteins, and exhibit fluorescence increases that enable protein expression to be imaged in living cells. These sensors provide a general strategy to image protein expression in single bacteria in real-time.
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spelling pubmed-37584212014-03-01 Imaging bacterial protein expression using genetically encoded sensors composed of RNA Song, Wenjiao Strack, Rita L. Jaffrey, Samie R. Nat Methods Article We show that the difficulties in imaging the dynamics of protein expression in live bacterial cells can be overcome using fluorescent sensors based on Spinach, an RNA that activates the fluorescence of a small-molecule fluorophore. These RNAs selectively bind target proteins, and exhibit fluorescence increases that enable protein expression to be imaged in living cells. These sensors provide a general strategy to image protein expression in single bacteria in real-time. 2013-07-21 2013-09 /pmc/articles/PMC3758421/ /pubmed/23872791 http://dx.doi.org/10.1038/nmeth.2568 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Song, Wenjiao
Strack, Rita L.
Jaffrey, Samie R.
Imaging bacterial protein expression using genetically encoded sensors composed of RNA
title Imaging bacterial protein expression using genetically encoded sensors composed of RNA
title_full Imaging bacterial protein expression using genetically encoded sensors composed of RNA
title_fullStr Imaging bacterial protein expression using genetically encoded sensors composed of RNA
title_full_unstemmed Imaging bacterial protein expression using genetically encoded sensors composed of RNA
title_short Imaging bacterial protein expression using genetically encoded sensors composed of RNA
title_sort imaging bacterial protein expression using genetically encoded sensors composed of rna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3758421/
https://www.ncbi.nlm.nih.gov/pubmed/23872791
http://dx.doi.org/10.1038/nmeth.2568
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