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Iron economy in Chlamydomonas reinhardtii
While research on iron nutrition in plants has largely focused on iron-uptake pathways, photosynthetic microbes such as the unicellular green alga Chlamydomonas reinhardtii provide excellent experimental systems for understanding iron metabolism at the subcellular level. Several paradigms in iron ho...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759009/ https://www.ncbi.nlm.nih.gov/pubmed/24032036 http://dx.doi.org/10.3389/fpls.2013.00337 |
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author | Glaesener, Anne G. Merchant, Sabeeha S. Blaby-Haas, Crysten E. |
author_facet | Glaesener, Anne G. Merchant, Sabeeha S. Blaby-Haas, Crysten E. |
author_sort | Glaesener, Anne G. |
collection | PubMed |
description | While research on iron nutrition in plants has largely focused on iron-uptake pathways, photosynthetic microbes such as the unicellular green alga Chlamydomonas reinhardtii provide excellent experimental systems for understanding iron metabolism at the subcellular level. Several paradigms in iron homeostasis have been established in this alga, including photosystem remodeling in the chloroplast and preferential retention of some pathways and key iron-dependent proteins in response to suboptimal iron supply. This review presents our current understanding of iron homeostasis in Chlamydomonas, with specific attention on characterized responses to changes in iron supply, like iron-deficiency. An overview of frequently used methods for the investigation of iron-responsive gene expression, physiology and metabolism is also provided, including preparation of media, the effect of cell size, cell density and strain choice on quantitative measurements and methods for the determination of metal content and assessing the effect of iron supply on photosynthetic performance. |
format | Online Article Text |
id | pubmed-3759009 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-37590092013-09-12 Iron economy in Chlamydomonas reinhardtii Glaesener, Anne G. Merchant, Sabeeha S. Blaby-Haas, Crysten E. Front Plant Sci Plant Science While research on iron nutrition in plants has largely focused on iron-uptake pathways, photosynthetic microbes such as the unicellular green alga Chlamydomonas reinhardtii provide excellent experimental systems for understanding iron metabolism at the subcellular level. Several paradigms in iron homeostasis have been established in this alga, including photosystem remodeling in the chloroplast and preferential retention of some pathways and key iron-dependent proteins in response to suboptimal iron supply. This review presents our current understanding of iron homeostasis in Chlamydomonas, with specific attention on characterized responses to changes in iron supply, like iron-deficiency. An overview of frequently used methods for the investigation of iron-responsive gene expression, physiology and metabolism is also provided, including preparation of media, the effect of cell size, cell density and strain choice on quantitative measurements and methods for the determination of metal content and assessing the effect of iron supply on photosynthetic performance. Frontiers Media S.A. 2013-09-02 /pmc/articles/PMC3759009/ /pubmed/24032036 http://dx.doi.org/10.3389/fpls.2013.00337 Text en Copyright © 2013 Glaesener, Merchant and Blaby-Haas. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Glaesener, Anne G. Merchant, Sabeeha S. Blaby-Haas, Crysten E. Iron economy in Chlamydomonas reinhardtii |
title | Iron economy in Chlamydomonas reinhardtii |
title_full | Iron economy in Chlamydomonas reinhardtii |
title_fullStr | Iron economy in Chlamydomonas reinhardtii |
title_full_unstemmed | Iron economy in Chlamydomonas reinhardtii |
title_short | Iron economy in Chlamydomonas reinhardtii |
title_sort | iron economy in chlamydomonas reinhardtii |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759009/ https://www.ncbi.nlm.nih.gov/pubmed/24032036 http://dx.doi.org/10.3389/fpls.2013.00337 |
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