A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology

Tools that allow for rapid, accurate and inexpensive assembly of multi-component combinatorial libraries of DNA for transformation into plants will accelerate the progress of synthetic biology research. Recent innovations in molecular cloning methods has vastly expanded the repertoire with which pla...

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Autores principales: Emami, Shahram, Yee, Muh-ching, Dinneny, José R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759027/
https://www.ncbi.nlm.nih.gov/pubmed/24032037
http://dx.doi.org/10.3389/fpls.2013.00339
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author Emami, Shahram
Yee, Muh-ching
Dinneny, José R.
author_facet Emami, Shahram
Yee, Muh-ching
Dinneny, José R.
author_sort Emami, Shahram
collection PubMed
description Tools that allow for rapid, accurate and inexpensive assembly of multi-component combinatorial libraries of DNA for transformation into plants will accelerate the progress of synthetic biology research. Recent innovations in molecular cloning methods has vastly expanded the repertoire with which plant biologists can engineer a transgene. Here we describe a new set of binary vectors for use in Agrobacterium-mediated plant transformation that utilizes the Golden-Gate Cloning approach. Our optimized protocol facilitates the rapid and inexpensive generation of multi-component transgenes for later introduction into plants.
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spelling pubmed-37590272013-09-12 A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology Emami, Shahram Yee, Muh-ching Dinneny, José R. Front Plant Sci Plant Science Tools that allow for rapid, accurate and inexpensive assembly of multi-component combinatorial libraries of DNA for transformation into plants will accelerate the progress of synthetic biology research. Recent innovations in molecular cloning methods has vastly expanded the repertoire with which plant biologists can engineer a transgene. Here we describe a new set of binary vectors for use in Agrobacterium-mediated plant transformation that utilizes the Golden-Gate Cloning approach. Our optimized protocol facilitates the rapid and inexpensive generation of multi-component transgenes for later introduction into plants. Frontiers Media S.A. 2013-09-02 /pmc/articles/PMC3759027/ /pubmed/24032037 http://dx.doi.org/10.3389/fpls.2013.00339 Text en Copyright © 2013 Emami, Yee and Dinneny. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Emami, Shahram
Yee, Muh-ching
Dinneny, José R.
A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology
title A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology
title_full A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology
title_fullStr A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology
title_full_unstemmed A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology
title_short A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology
title_sort robust family of golden gate agrobacterium vectors for plant synthetic biology
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759027/
https://www.ncbi.nlm.nih.gov/pubmed/24032037
http://dx.doi.org/10.3389/fpls.2013.00339
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