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β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells

Estrogen receptor-alpha (ERα) is a key factor in the development of breast cancer in humans. The expression and activity of ERα is regulated by a multitude of intracellular and extracellular signals. Here we show a cross-talk between β-catenin and ERα in human breast cancer cells. Knockdown of β-cat...

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Autores principales: Gupta, Nibedita, Schmitt, Fee, Grebhardt, Sina, Mayer, Doris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759182/
https://www.ncbi.nlm.nih.gov/pubmed/24212942
http://dx.doi.org/10.3390/cancers3032990
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author Gupta, Nibedita
Schmitt, Fee
Grebhardt, Sina
Mayer, Doris
author_facet Gupta, Nibedita
Schmitt, Fee
Grebhardt, Sina
Mayer, Doris
author_sort Gupta, Nibedita
collection PubMed
description Estrogen receptor-alpha (ERα) is a key factor in the development of breast cancer in humans. The expression and activity of ERα is regulated by a multitude of intracellular and extracellular signals. Here we show a cross-talk between β-catenin and ERα in human breast cancer cells. Knockdown of β-catenin by RNAi resulted in significant reduction of ERα mRNA and/or protein levels in MCF-7, T-47D, and BT-474 breast cancer cells and in significant reduction of estradiol-induced expression of the ERα target genes pS2 and GREB1. In addition β-catenin silencing resulted in significant decrease of growth of MCF-7 cells both in the absence and presence of estradiol. β-catenin and ERα could not be co-immunoprecipitated by ERα antibodies from lysates of E2-treated or untreated cells suggesting lack of direct physical interaction. It is concluded that β-catenin is a positive regulator of ERα mRNA and protein expression.
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spelling pubmed-37591822013-09-04 β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells Gupta, Nibedita Schmitt, Fee Grebhardt, Sina Mayer, Doris Cancers (Basel) Article Estrogen receptor-alpha (ERα) is a key factor in the development of breast cancer in humans. The expression and activity of ERα is regulated by a multitude of intracellular and extracellular signals. Here we show a cross-talk between β-catenin and ERα in human breast cancer cells. Knockdown of β-catenin by RNAi resulted in significant reduction of ERα mRNA and/or protein levels in MCF-7, T-47D, and BT-474 breast cancer cells and in significant reduction of estradiol-induced expression of the ERα target genes pS2 and GREB1. In addition β-catenin silencing resulted in significant decrease of growth of MCF-7 cells both in the absence and presence of estradiol. β-catenin and ERα could not be co-immunoprecipitated by ERα antibodies from lysates of E2-treated or untreated cells suggesting lack of direct physical interaction. It is concluded that β-catenin is a positive regulator of ERα mRNA and protein expression. Molecular Diversity Preservation International (MDPI) 2011-07-22 /pmc/articles/PMC3759182/ /pubmed/24212942 http://dx.doi.org/10.3390/cancers3032990 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Gupta, Nibedita
Schmitt, Fee
Grebhardt, Sina
Mayer, Doris
β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells
title β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells
title_full β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells
title_fullStr β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells
title_full_unstemmed β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells
title_short β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells
title_sort β-catenin is a positive regulator of estrogen receptor-α function in breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759182/
https://www.ncbi.nlm.nih.gov/pubmed/24212942
http://dx.doi.org/10.3390/cancers3032990
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