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β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells
Estrogen receptor-alpha (ERα) is a key factor in the development of breast cancer in humans. The expression and activity of ERα is regulated by a multitude of intracellular and extracellular signals. Here we show a cross-talk between β-catenin and ERα in human breast cancer cells. Knockdown of β-cat...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759182/ https://www.ncbi.nlm.nih.gov/pubmed/24212942 http://dx.doi.org/10.3390/cancers3032990 |
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author | Gupta, Nibedita Schmitt, Fee Grebhardt, Sina Mayer, Doris |
author_facet | Gupta, Nibedita Schmitt, Fee Grebhardt, Sina Mayer, Doris |
author_sort | Gupta, Nibedita |
collection | PubMed |
description | Estrogen receptor-alpha (ERα) is a key factor in the development of breast cancer in humans. The expression and activity of ERα is regulated by a multitude of intracellular and extracellular signals. Here we show a cross-talk between β-catenin and ERα in human breast cancer cells. Knockdown of β-catenin by RNAi resulted in significant reduction of ERα mRNA and/or protein levels in MCF-7, T-47D, and BT-474 breast cancer cells and in significant reduction of estradiol-induced expression of the ERα target genes pS2 and GREB1. In addition β-catenin silencing resulted in significant decrease of growth of MCF-7 cells both in the absence and presence of estradiol. β-catenin and ERα could not be co-immunoprecipitated by ERα antibodies from lysates of E2-treated or untreated cells suggesting lack of direct physical interaction. It is concluded that β-catenin is a positive regulator of ERα mRNA and protein expression. |
format | Online Article Text |
id | pubmed-3759182 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-37591822013-09-04 β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells Gupta, Nibedita Schmitt, Fee Grebhardt, Sina Mayer, Doris Cancers (Basel) Article Estrogen receptor-alpha (ERα) is a key factor in the development of breast cancer in humans. The expression and activity of ERα is regulated by a multitude of intracellular and extracellular signals. Here we show a cross-talk between β-catenin and ERα in human breast cancer cells. Knockdown of β-catenin by RNAi resulted in significant reduction of ERα mRNA and/or protein levels in MCF-7, T-47D, and BT-474 breast cancer cells and in significant reduction of estradiol-induced expression of the ERα target genes pS2 and GREB1. In addition β-catenin silencing resulted in significant decrease of growth of MCF-7 cells both in the absence and presence of estradiol. β-catenin and ERα could not be co-immunoprecipitated by ERα antibodies from lysates of E2-treated or untreated cells suggesting lack of direct physical interaction. It is concluded that β-catenin is a positive regulator of ERα mRNA and protein expression. Molecular Diversity Preservation International (MDPI) 2011-07-22 /pmc/articles/PMC3759182/ /pubmed/24212942 http://dx.doi.org/10.3390/cancers3032990 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Gupta, Nibedita Schmitt, Fee Grebhardt, Sina Mayer, Doris β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells |
title | β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells |
title_full | β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells |
title_fullStr | β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells |
title_full_unstemmed | β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells |
title_short | β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells |
title_sort | β-catenin is a positive regulator of estrogen receptor-α function in breast cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759182/ https://www.ncbi.nlm.nih.gov/pubmed/24212942 http://dx.doi.org/10.3390/cancers3032990 |
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