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An Efficient 3D Cell Culture Method on Biomimetic Nanostructured Grids
Current techniques of in vitro cell cultures are able to mimic the in vivo environment only to a limited extent, as they enable cells to grow only in two dimensions. Therefore cell culture approaches should rely on scaffolds that provide support comparable to the extracellular matrix. Here we demons...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759432/ https://www.ncbi.nlm.nih.gov/pubmed/24023793 http://dx.doi.org/10.1371/journal.pone.0072936 |
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author | Wolun-Cholewa, Maria Langer, Krzysztof Szymanowski, Krzysztof Glodek, Aleksandra Jankowska, Anna Warchol, Wojciech Langer, Jerzy |
author_facet | Wolun-Cholewa, Maria Langer, Krzysztof Szymanowski, Krzysztof Glodek, Aleksandra Jankowska, Anna Warchol, Wojciech Langer, Jerzy |
author_sort | Wolun-Cholewa, Maria |
collection | PubMed |
description | Current techniques of in vitro cell cultures are able to mimic the in vivo environment only to a limited extent, as they enable cells to grow only in two dimensions. Therefore cell culture approaches should rely on scaffolds that provide support comparable to the extracellular matrix. Here we demonstrate the advantages of novel nanostructured three-dimensional grids fabricated using electro-spinning technique, as scaffolds for cultures of neoplastic cells. The results of the study show that the fibers allow for a dynamic growth of HeLa cells, which form multi-layer structures of symmetrical and spherical character. This indicates that the applied scaffolds are nontoxic and allow proper flow of oxygen, nutrients, and growth factors. In addition, grids have been proven to be useful in in situ examination of cells ultrastructure. |
format | Online Article Text |
id | pubmed-3759432 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37594322013-09-10 An Efficient 3D Cell Culture Method on Biomimetic Nanostructured Grids Wolun-Cholewa, Maria Langer, Krzysztof Szymanowski, Krzysztof Glodek, Aleksandra Jankowska, Anna Warchol, Wojciech Langer, Jerzy PLoS One Research Article Current techniques of in vitro cell cultures are able to mimic the in vivo environment only to a limited extent, as they enable cells to grow only in two dimensions. Therefore cell culture approaches should rely on scaffolds that provide support comparable to the extracellular matrix. Here we demonstrate the advantages of novel nanostructured three-dimensional grids fabricated using electro-spinning technique, as scaffolds for cultures of neoplastic cells. The results of the study show that the fibers allow for a dynamic growth of HeLa cells, which form multi-layer structures of symmetrical and spherical character. This indicates that the applied scaffolds are nontoxic and allow proper flow of oxygen, nutrients, and growth factors. In addition, grids have been proven to be useful in in situ examination of cells ultrastructure. Public Library of Science 2013-09-02 /pmc/articles/PMC3759432/ /pubmed/24023793 http://dx.doi.org/10.1371/journal.pone.0072936 Text en © 2013 Wolun-Cholewa et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wolun-Cholewa, Maria Langer, Krzysztof Szymanowski, Krzysztof Glodek, Aleksandra Jankowska, Anna Warchol, Wojciech Langer, Jerzy An Efficient 3D Cell Culture Method on Biomimetic Nanostructured Grids |
title | An Efficient 3D Cell Culture Method on Biomimetic Nanostructured Grids |
title_full | An Efficient 3D Cell Culture Method on Biomimetic Nanostructured Grids |
title_fullStr | An Efficient 3D Cell Culture Method on Biomimetic Nanostructured Grids |
title_full_unstemmed | An Efficient 3D Cell Culture Method on Biomimetic Nanostructured Grids |
title_short | An Efficient 3D Cell Culture Method on Biomimetic Nanostructured Grids |
title_sort | efficient 3d cell culture method on biomimetic nanostructured grids |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759432/ https://www.ncbi.nlm.nih.gov/pubmed/24023793 http://dx.doi.org/10.1371/journal.pone.0072936 |
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