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Complete Genome Analysis of a Haemophilus parasuis Serovar 12 Strain from China

Haemophilus parasuis is the etiological agent of Glässer's disease in pigs and 15 standard serovars were identified. The widespread disease causes great economic loss in the swine industry worldwide. Aiming to investigate the differences in genome composition and functions among various strains...

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Autores principales: Li, Yufeng, Kwok, Amy H. Y., Jiang, Jingwei, Zou, Yao, Zheng, Fangyuan, Chen, Pan, Hou, Chengcai, Leung, Frederick C., Jiang, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759607/
https://www.ncbi.nlm.nih.gov/pubmed/24023711
http://dx.doi.org/10.1371/journal.pone.0068350
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author Li, Yufeng
Kwok, Amy H. Y.
Jiang, Jingwei
Zou, Yao
Zheng, Fangyuan
Chen, Pan
Hou, Chengcai
Leung, Frederick C.
Jiang, Ping
author_facet Li, Yufeng
Kwok, Amy H. Y.
Jiang, Jingwei
Zou, Yao
Zheng, Fangyuan
Chen, Pan
Hou, Chengcai
Leung, Frederick C.
Jiang, Ping
author_sort Li, Yufeng
collection PubMed
description Haemophilus parasuis is the etiological agent of Glässer's disease in pigs and 15 standard serovars were identified. The widespread disease causes great economic loss in the swine industry worldwide. Aiming to investigate the differences in genome composition and functions among various strains, a highly virulent strain ZJ0906 of H. parasuis serovar 12 from China was analyzed and compared with serovar 5 SH0165. Strain ZJ0906 genome is 2,324,740 base pairs with 40.06% genomic GC content. It contains 2,484 open reading frames (ORF) predicted by Glimmer 3.02, of which 2,352 (∼94.7%) were annotated by NCBI nr blast, 1,745 by COG database and 1,829 by KEGG database. 109 potential virulence factors were annotated in strain ZJ0906 and 3 of which are potentially related to antibiotic resistance. Strain ZJ0906 genome is ∼55 kilobases longer than SH0165 genome, with an extra 211 predicted ORFs. VFDB, ARDB, and PAIDB blast searches showed that ZJ0906 and SH0165 shared a nearly identical panel of potential virulence factors, drug resistant genes and four PAI-like regions which showed high homology to Enterococcus, Escherichia and Salmonella. Synteny analysis showed that gene rearrangements are frequent between the two strains, which may lead to variations in pathogenicity and cross-protection among serovars. KEGG pathway analyses showed strain ZJ0906 shared similar metabolic pathways to strain SH0165. Molecular identification of these genomic elements and potential virulence factors pave the way to the better understanding of mechanisms underlying metabolic capabilities and pathogenicity of H. parasuis and prospective vaccine targets besides the widely used method of inactivated bacteria.
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spelling pubmed-37596072013-09-10 Complete Genome Analysis of a Haemophilus parasuis Serovar 12 Strain from China Li, Yufeng Kwok, Amy H. Y. Jiang, Jingwei Zou, Yao Zheng, Fangyuan Chen, Pan Hou, Chengcai Leung, Frederick C. Jiang, Ping PLoS One Research Article Haemophilus parasuis is the etiological agent of Glässer's disease in pigs and 15 standard serovars were identified. The widespread disease causes great economic loss in the swine industry worldwide. Aiming to investigate the differences in genome composition and functions among various strains, a highly virulent strain ZJ0906 of H. parasuis serovar 12 from China was analyzed and compared with serovar 5 SH0165. Strain ZJ0906 genome is 2,324,740 base pairs with 40.06% genomic GC content. It contains 2,484 open reading frames (ORF) predicted by Glimmer 3.02, of which 2,352 (∼94.7%) were annotated by NCBI nr blast, 1,745 by COG database and 1,829 by KEGG database. 109 potential virulence factors were annotated in strain ZJ0906 and 3 of which are potentially related to antibiotic resistance. Strain ZJ0906 genome is ∼55 kilobases longer than SH0165 genome, with an extra 211 predicted ORFs. VFDB, ARDB, and PAIDB blast searches showed that ZJ0906 and SH0165 shared a nearly identical panel of potential virulence factors, drug resistant genes and four PAI-like regions which showed high homology to Enterococcus, Escherichia and Salmonella. Synteny analysis showed that gene rearrangements are frequent between the two strains, which may lead to variations in pathogenicity and cross-protection among serovars. KEGG pathway analyses showed strain ZJ0906 shared similar metabolic pathways to strain SH0165. Molecular identification of these genomic elements and potential virulence factors pave the way to the better understanding of mechanisms underlying metabolic capabilities and pathogenicity of H. parasuis and prospective vaccine targets besides the widely used method of inactivated bacteria. Public Library of Science 2013-09-02 /pmc/articles/PMC3759607/ /pubmed/24023711 http://dx.doi.org/10.1371/journal.pone.0068350 Text en © 2013 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Yufeng
Kwok, Amy H. Y.
Jiang, Jingwei
Zou, Yao
Zheng, Fangyuan
Chen, Pan
Hou, Chengcai
Leung, Frederick C.
Jiang, Ping
Complete Genome Analysis of a Haemophilus parasuis Serovar 12 Strain from China
title Complete Genome Analysis of a Haemophilus parasuis Serovar 12 Strain from China
title_full Complete Genome Analysis of a Haemophilus parasuis Serovar 12 Strain from China
title_fullStr Complete Genome Analysis of a Haemophilus parasuis Serovar 12 Strain from China
title_full_unstemmed Complete Genome Analysis of a Haemophilus parasuis Serovar 12 Strain from China
title_short Complete Genome Analysis of a Haemophilus parasuis Serovar 12 Strain from China
title_sort complete genome analysis of a haemophilus parasuis serovar 12 strain from china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759607/
https://www.ncbi.nlm.nih.gov/pubmed/24023711
http://dx.doi.org/10.1371/journal.pone.0068350
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