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Neuron Biomechanics Probed by Atomic Force Microscopy
Mechanical interactions play a key role in many processes associated with neuronal growth and development. Over the last few years there has been significant progress in our understanding of the role played by the substrate stiffness in neuronal growth, of the cell-substrate adhesion forces, of the...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759903/ https://www.ncbi.nlm.nih.gov/pubmed/23921683 http://dx.doi.org/10.3390/ijms140816124 |
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author | Spedden, Elise Staii, Cristian |
author_facet | Spedden, Elise Staii, Cristian |
author_sort | Spedden, Elise |
collection | PubMed |
description | Mechanical interactions play a key role in many processes associated with neuronal growth and development. Over the last few years there has been significant progress in our understanding of the role played by the substrate stiffness in neuronal growth, of the cell-substrate adhesion forces, of the generation of traction forces during axonal elongation, and of the relationships between the neuron soma elastic properties and its health. The particular capabilities of the Atomic Force Microscope (AFM), such as high spatial resolution, high degree of control over the magnitude and orientation of the applied forces, minimal sample damage, and the ability to image and interact with cells in physiologically relevant conditions make this technique particularly suitable for measuring mechanical properties of living neuronal cells. This article reviews recent advances on using the AFM for studying neuronal biomechanics, provides an overview about the state-of-the-art measurements, and suggests directions for future applications. |
format | Online Article Text |
id | pubmed-3759903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-37599032013-09-03 Neuron Biomechanics Probed by Atomic Force Microscopy Spedden, Elise Staii, Cristian Int J Mol Sci Review Mechanical interactions play a key role in many processes associated with neuronal growth and development. Over the last few years there has been significant progress in our understanding of the role played by the substrate stiffness in neuronal growth, of the cell-substrate adhesion forces, of the generation of traction forces during axonal elongation, and of the relationships between the neuron soma elastic properties and its health. The particular capabilities of the Atomic Force Microscope (AFM), such as high spatial resolution, high degree of control over the magnitude and orientation of the applied forces, minimal sample damage, and the ability to image and interact with cells in physiologically relevant conditions make this technique particularly suitable for measuring mechanical properties of living neuronal cells. This article reviews recent advances on using the AFM for studying neuronal biomechanics, provides an overview about the state-of-the-art measurements, and suggests directions for future applications. Molecular Diversity Preservation International (MDPI) 2013-08-05 /pmc/articles/PMC3759903/ /pubmed/23921683 http://dx.doi.org/10.3390/ijms140816124 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Review Spedden, Elise Staii, Cristian Neuron Biomechanics Probed by Atomic Force Microscopy |
title | Neuron Biomechanics Probed by Atomic Force Microscopy |
title_full | Neuron Biomechanics Probed by Atomic Force Microscopy |
title_fullStr | Neuron Biomechanics Probed by Atomic Force Microscopy |
title_full_unstemmed | Neuron Biomechanics Probed by Atomic Force Microscopy |
title_short | Neuron Biomechanics Probed by Atomic Force Microscopy |
title_sort | neuron biomechanics probed by atomic force microscopy |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759903/ https://www.ncbi.nlm.nih.gov/pubmed/23921683 http://dx.doi.org/10.3390/ijms140816124 |
work_keys_str_mv | AT speddenelise neuronbiomechanicsprobedbyatomicforcemicroscopy AT staiicristian neuronbiomechanicsprobedbyatomicforcemicroscopy |