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The Efficacy of the Quorum Sensing Inhibitor FS8 and Tigecycline in Preventing Prosthesis Biofilm in an Animal Model of Staphylococcal Infection

We investigated the efficacy of tigecycline and FS8, alone or combined, in preventing prosthesis biofilm in a rat model of staphylococcal vascular graft infection. Graft infections were established in the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses followe...

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Autores principales: Simonetti, Oriana, Cirioni, Oscar, Mocchegiani, Federico, Cacciatore, Ivana, Silvestri, Carmela, Baldassarre, Leonardo, Orlando, Fiorenza, Castelli, Pamela, Provinciali, Mauro, Vivarelli, Marco, Fornasari, Erika, Giacometti, Andrea, Offidani, Annamaria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759913/
https://www.ncbi.nlm.nih.gov/pubmed/23965956
http://dx.doi.org/10.3390/ijms140816321
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author Simonetti, Oriana
Cirioni, Oscar
Mocchegiani, Federico
Cacciatore, Ivana
Silvestri, Carmela
Baldassarre, Leonardo
Orlando, Fiorenza
Castelli, Pamela
Provinciali, Mauro
Vivarelli, Marco
Fornasari, Erika
Giacometti, Andrea
Offidani, Annamaria
author_facet Simonetti, Oriana
Cirioni, Oscar
Mocchegiani, Federico
Cacciatore, Ivana
Silvestri, Carmela
Baldassarre, Leonardo
Orlando, Fiorenza
Castelli, Pamela
Provinciali, Mauro
Vivarelli, Marco
Fornasari, Erika
Giacometti, Andrea
Offidani, Annamaria
author_sort Simonetti, Oriana
collection PubMed
description We investigated the efficacy of tigecycline and FS8, alone or combined, in preventing prosthesis biofilm in a rat model of staphylococcal vascular graft infection. Graft infections were established in the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses followed by topical inoculation with 2 × 10(7) colony-forming units of Staphylococcus aureus, strain Smith diffuse. The study included a control group, a contaminated group that did not receive any antibiotic prophylaxis, and three contaminated groups that received: (i) intraperitoneal tigecycline, (ii) FS8-soaked graft, and (iii) tigecycline plus FS8-soaked graft, respectively. Each group included 15 animals. The infection burden was evaluated by using sonication and quantitative agar culture. Moreover, an in vitro binding-study was performed to quantify the how much FS8 was coated to the surface of the prosthesis. Tigecycline, combined with FS8, against the adherent bacteria showed MICs (2.00 mg/L) and MBCs (4.00 mg/L) four-fold lower with respect to tigecycline alone in in vitro studies. The rat groups treated with tigecycline showed the lowest bacterial numbers (4.4 × 10(4) ± 1.2 × 10(4) CFU/mL). The FS8-treated group showed a good activity and significant differences compared to control group with bacterial numbers of 6.8 × 10(4) ± 2.0 × 10(4) CFU/mL. A stronger inhibition of bacterial growth was observed in rats treated with a combined FS8 and tigecycline therapy than in those that were singly treated with bacterial numbers of 10(1) CFU/mL graft. In conclusion, the ability to affect biofilm formation as well, its property to be an antibiotic enhancer suggests FS8 as alternative or additional agent to use in conjunction with conventional antimicrobial for prevention of staphylococcal biofilm related infection.
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spelling pubmed-37599132013-09-03 The Efficacy of the Quorum Sensing Inhibitor FS8 and Tigecycline in Preventing Prosthesis Biofilm in an Animal Model of Staphylococcal Infection Simonetti, Oriana Cirioni, Oscar Mocchegiani, Federico Cacciatore, Ivana Silvestri, Carmela Baldassarre, Leonardo Orlando, Fiorenza Castelli, Pamela Provinciali, Mauro Vivarelli, Marco Fornasari, Erika Giacometti, Andrea Offidani, Annamaria Int J Mol Sci Article We investigated the efficacy of tigecycline and FS8, alone or combined, in preventing prosthesis biofilm in a rat model of staphylococcal vascular graft infection. Graft infections were established in the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses followed by topical inoculation with 2 × 10(7) colony-forming units of Staphylococcus aureus, strain Smith diffuse. The study included a control group, a contaminated group that did not receive any antibiotic prophylaxis, and three contaminated groups that received: (i) intraperitoneal tigecycline, (ii) FS8-soaked graft, and (iii) tigecycline plus FS8-soaked graft, respectively. Each group included 15 animals. The infection burden was evaluated by using sonication and quantitative agar culture. Moreover, an in vitro binding-study was performed to quantify the how much FS8 was coated to the surface of the prosthesis. Tigecycline, combined with FS8, against the adherent bacteria showed MICs (2.00 mg/L) and MBCs (4.00 mg/L) four-fold lower with respect to tigecycline alone in in vitro studies. The rat groups treated with tigecycline showed the lowest bacterial numbers (4.4 × 10(4) ± 1.2 × 10(4) CFU/mL). The FS8-treated group showed a good activity and significant differences compared to control group with bacterial numbers of 6.8 × 10(4) ± 2.0 × 10(4) CFU/mL. A stronger inhibition of bacterial growth was observed in rats treated with a combined FS8 and tigecycline therapy than in those that were singly treated with bacterial numbers of 10(1) CFU/mL graft. In conclusion, the ability to affect biofilm formation as well, its property to be an antibiotic enhancer suggests FS8 as alternative or additional agent to use in conjunction with conventional antimicrobial for prevention of staphylococcal biofilm related infection. Molecular Diversity Preservation International (MDPI) 2013-08-07 /pmc/articles/PMC3759913/ /pubmed/23965956 http://dx.doi.org/10.3390/ijms140816321 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Simonetti, Oriana
Cirioni, Oscar
Mocchegiani, Federico
Cacciatore, Ivana
Silvestri, Carmela
Baldassarre, Leonardo
Orlando, Fiorenza
Castelli, Pamela
Provinciali, Mauro
Vivarelli, Marco
Fornasari, Erika
Giacometti, Andrea
Offidani, Annamaria
The Efficacy of the Quorum Sensing Inhibitor FS8 and Tigecycline in Preventing Prosthesis Biofilm in an Animal Model of Staphylococcal Infection
title The Efficacy of the Quorum Sensing Inhibitor FS8 and Tigecycline in Preventing Prosthesis Biofilm in an Animal Model of Staphylococcal Infection
title_full The Efficacy of the Quorum Sensing Inhibitor FS8 and Tigecycline in Preventing Prosthesis Biofilm in an Animal Model of Staphylococcal Infection
title_fullStr The Efficacy of the Quorum Sensing Inhibitor FS8 and Tigecycline in Preventing Prosthesis Biofilm in an Animal Model of Staphylococcal Infection
title_full_unstemmed The Efficacy of the Quorum Sensing Inhibitor FS8 and Tigecycline in Preventing Prosthesis Biofilm in an Animal Model of Staphylococcal Infection
title_short The Efficacy of the Quorum Sensing Inhibitor FS8 and Tigecycline in Preventing Prosthesis Biofilm in an Animal Model of Staphylococcal Infection
title_sort efficacy of the quorum sensing inhibitor fs8 and tigecycline in preventing prosthesis biofilm in an animal model of staphylococcal infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759913/
https://www.ncbi.nlm.nih.gov/pubmed/23965956
http://dx.doi.org/10.3390/ijms140816321
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