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Effects of Reduced Prolamin on Seed Storage Protein Composition and the Nutritional Quality of Rice

Rice seed storage proteins accumulate in two types of protein body (PB-I and PB-II) that are nutrient sources for animals. PB-I is indigestible and negatively affects rice protein quality. To improve the nutritional value of rice seeds we are aiming to engineer the composition and accumulation of en...

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Autores principales: Kim, Hyun-Jung, Lee, Jong-Yeol, Yoon, Ung-Han, Lim, Sun-Hyung, Kim, Young-Mi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759952/
https://www.ncbi.nlm.nih.gov/pubmed/23965973
http://dx.doi.org/10.3390/ijms140817073
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author Kim, Hyun-Jung
Lee, Jong-Yeol
Yoon, Ung-Han
Lim, Sun-Hyung
Kim, Young-Mi
author_facet Kim, Hyun-Jung
Lee, Jong-Yeol
Yoon, Ung-Han
Lim, Sun-Hyung
Kim, Young-Mi
author_sort Kim, Hyun-Jung
collection PubMed
description Rice seed storage proteins accumulate in two types of protein body (PB-I and PB-II) that are nutrient sources for animals. PB-I is indigestible and negatively affects rice protein quality. To improve the nutritional value of rice seeds we are aiming to engineer the composition and accumulation of endogenous seed storage proteins. In this study we generated transgenic rice plants in which 13 kD prolamin genes were suppressed by RNA interference (13 kD pro-RNAi). Analysis based on qRT-PCR confirmed that the targeted 13 kD prolamins were markedly suppressed, and were compensated for by an increase in other storage proteins including 10 kD prolamin, glutelins, and chaperone proteins. The storage protein profiles further revealed that the levels of 13 kD prolamins were significantly reduced, while that of the glutelin precursor was slightly increased and the remaining storage proteins did not change. Amino acid analysis showed that the reduction of 13 kD prolamins resulted in a 28% increase in the lysine content relative to the wild type, indicating that the 13 kD pro-RNAi rice seeds are more nutritious. Furthermore, a reduction in the levels of 13 kD prolamins resulted in abnormal formation of PB-I, which was small and had no lamellar structure. These results suggest that alteration of prolamins can contribute to improving the nutritional quality of rice.
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spelling pubmed-37599522013-09-03 Effects of Reduced Prolamin on Seed Storage Protein Composition and the Nutritional Quality of Rice Kim, Hyun-Jung Lee, Jong-Yeol Yoon, Ung-Han Lim, Sun-Hyung Kim, Young-Mi Int J Mol Sci Article Rice seed storage proteins accumulate in two types of protein body (PB-I and PB-II) that are nutrient sources for animals. PB-I is indigestible and negatively affects rice protein quality. To improve the nutritional value of rice seeds we are aiming to engineer the composition and accumulation of endogenous seed storage proteins. In this study we generated transgenic rice plants in which 13 kD prolamin genes were suppressed by RNA interference (13 kD pro-RNAi). Analysis based on qRT-PCR confirmed that the targeted 13 kD prolamins were markedly suppressed, and were compensated for by an increase in other storage proteins including 10 kD prolamin, glutelins, and chaperone proteins. The storage protein profiles further revealed that the levels of 13 kD prolamins were significantly reduced, while that of the glutelin precursor was slightly increased and the remaining storage proteins did not change. Amino acid analysis showed that the reduction of 13 kD prolamins resulted in a 28% increase in the lysine content relative to the wild type, indicating that the 13 kD pro-RNAi rice seeds are more nutritious. Furthermore, a reduction in the levels of 13 kD prolamins resulted in abnormal formation of PB-I, which was small and had no lamellar structure. These results suggest that alteration of prolamins can contribute to improving the nutritional quality of rice. Molecular Diversity Preservation International (MDPI) 2013-08-19 /pmc/articles/PMC3759952/ /pubmed/23965973 http://dx.doi.org/10.3390/ijms140817073 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Kim, Hyun-Jung
Lee, Jong-Yeol
Yoon, Ung-Han
Lim, Sun-Hyung
Kim, Young-Mi
Effects of Reduced Prolamin on Seed Storage Protein Composition and the Nutritional Quality of Rice
title Effects of Reduced Prolamin on Seed Storage Protein Composition and the Nutritional Quality of Rice
title_full Effects of Reduced Prolamin on Seed Storage Protein Composition and the Nutritional Quality of Rice
title_fullStr Effects of Reduced Prolamin on Seed Storage Protein Composition and the Nutritional Quality of Rice
title_full_unstemmed Effects of Reduced Prolamin on Seed Storage Protein Composition and the Nutritional Quality of Rice
title_short Effects of Reduced Prolamin on Seed Storage Protein Composition and the Nutritional Quality of Rice
title_sort effects of reduced prolamin on seed storage protein composition and the nutritional quality of rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759952/
https://www.ncbi.nlm.nih.gov/pubmed/23965973
http://dx.doi.org/10.3390/ijms140817073
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