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Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line
This study was conducted to evaluate the effect of mesenchymal stem cells (MSCs) and a novel curcumin derivative (NCD) on HepG2 cells (hepatoma cell line) and to investigate their effect on Notch1 signaling pathway target genes. HepG2 cells were divided into HepG2 control group, HepG2 cells treated...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760179/ https://www.ncbi.nlm.nih.gov/pubmed/24024180 http://dx.doi.org/10.1155/2013/129629 |
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author | Abdel Aziz, Mohamed Talaat Khaled, Hussien Mostafa El Hindawi, Ali Roshdy, Nagwa Kamal Rashed, Laila A. Sabry, Dina Hassouna, Amira A. Taha, Fatma Ali, Walaa Ibrahim |
author_facet | Abdel Aziz, Mohamed Talaat Khaled, Hussien Mostafa El Hindawi, Ali Roshdy, Nagwa Kamal Rashed, Laila A. Sabry, Dina Hassouna, Amira A. Taha, Fatma Ali, Walaa Ibrahim |
author_sort | Abdel Aziz, Mohamed Talaat |
collection | PubMed |
description | This study was conducted to evaluate the effect of mesenchymal stem cells (MSCs) and a novel curcumin derivative (NCD) on HepG2 cells (hepatoma cell line) and to investigate their effect on Notch1 signaling pathway target genes. HepG2 cells were divided into HepG2 control group, HepG2 cells treated with MSC conditioned medium (MSCs CM), HepG2 cells treated with a NCD, HepG2 cells treated with MSCs CM and NCD, and HepG2 cells treated with MSCs CM (CM of MSCs pretreated with a NCD). Expression of Notch1, Hes1, VEGF, and cyclin D1 was assessed by real-time, reverse transcription-polymerase chain reaction (RT-PCR) in HepG2 cells. In addition, HepG2 proliferation assay was performed in all groups. Notch1 and its target genes (Hes1 and cyclin D1) were downregulated in all treated groups with more suppressive effect in the groups treated with both MSCs and NCD. Also, treated HepG2 cells showed significant decrease in cell proliferation rate. These data suggest that modulation of Notch1 signaling pathway by MSCs and/or NCD can be considered as a therapeutic target in HCC. |
format | Online Article Text |
id | pubmed-3760179 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-37601792013-09-10 Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line Abdel Aziz, Mohamed Talaat Khaled, Hussien Mostafa El Hindawi, Ali Roshdy, Nagwa Kamal Rashed, Laila A. Sabry, Dina Hassouna, Amira A. Taha, Fatma Ali, Walaa Ibrahim Biomed Res Int Research Article This study was conducted to evaluate the effect of mesenchymal stem cells (MSCs) and a novel curcumin derivative (NCD) on HepG2 cells (hepatoma cell line) and to investigate their effect on Notch1 signaling pathway target genes. HepG2 cells were divided into HepG2 control group, HepG2 cells treated with MSC conditioned medium (MSCs CM), HepG2 cells treated with a NCD, HepG2 cells treated with MSCs CM and NCD, and HepG2 cells treated with MSCs CM (CM of MSCs pretreated with a NCD). Expression of Notch1, Hes1, VEGF, and cyclin D1 was assessed by real-time, reverse transcription-polymerase chain reaction (RT-PCR) in HepG2 cells. In addition, HepG2 proliferation assay was performed in all groups. Notch1 and its target genes (Hes1 and cyclin D1) were downregulated in all treated groups with more suppressive effect in the groups treated with both MSCs and NCD. Also, treated HepG2 cells showed significant decrease in cell proliferation rate. These data suggest that modulation of Notch1 signaling pathway by MSCs and/or NCD can be considered as a therapeutic target in HCC. Hindawi Publishing Corporation 2013 2013-08-19 /pmc/articles/PMC3760179/ /pubmed/24024180 http://dx.doi.org/10.1155/2013/129629 Text en Copyright © 2013 Mohamed Talaat Abdel Aziz et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Abdel Aziz, Mohamed Talaat Khaled, Hussien Mostafa El Hindawi, Ali Roshdy, Nagwa Kamal Rashed, Laila A. Sabry, Dina Hassouna, Amira A. Taha, Fatma Ali, Walaa Ibrahim Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line |
title | Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line |
title_full | Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line |
title_fullStr | Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line |
title_full_unstemmed | Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line |
title_short | Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line |
title_sort | effect of mesenchymal stem cells and a novel curcumin derivative on notch1 signaling in hepatoma cell line |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760179/ https://www.ncbi.nlm.nih.gov/pubmed/24024180 http://dx.doi.org/10.1155/2013/129629 |
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