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Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line

This study was conducted to evaluate the effect of mesenchymal stem cells (MSCs) and a novel curcumin derivative (NCD) on HepG2 cells (hepatoma cell line) and to investigate their effect on Notch1 signaling pathway target genes. HepG2 cells were divided into HepG2 control group, HepG2 cells treated...

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Autores principales: Abdel Aziz, Mohamed Talaat, Khaled, Hussien Mostafa, El Hindawi, Ali, Roshdy, Nagwa Kamal, Rashed, Laila A., Sabry, Dina, Hassouna, Amira A., Taha, Fatma, Ali, Walaa Ibrahim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760179/
https://www.ncbi.nlm.nih.gov/pubmed/24024180
http://dx.doi.org/10.1155/2013/129629
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author Abdel Aziz, Mohamed Talaat
Khaled, Hussien Mostafa
El Hindawi, Ali
Roshdy, Nagwa Kamal
Rashed, Laila A.
Sabry, Dina
Hassouna, Amira A.
Taha, Fatma
Ali, Walaa Ibrahim
author_facet Abdel Aziz, Mohamed Talaat
Khaled, Hussien Mostafa
El Hindawi, Ali
Roshdy, Nagwa Kamal
Rashed, Laila A.
Sabry, Dina
Hassouna, Amira A.
Taha, Fatma
Ali, Walaa Ibrahim
author_sort Abdel Aziz, Mohamed Talaat
collection PubMed
description This study was conducted to evaluate the effect of mesenchymal stem cells (MSCs) and a novel curcumin derivative (NCD) on HepG2 cells (hepatoma cell line) and to investigate their effect on Notch1 signaling pathway target genes. HepG2 cells were divided into HepG2 control group, HepG2 cells treated with MSC conditioned medium (MSCs CM), HepG2 cells treated with a NCD, HepG2 cells treated with MSCs CM and NCD, and HepG2 cells treated with MSCs CM (CM of MSCs pretreated with a NCD). Expression of Notch1, Hes1, VEGF, and cyclin D1 was assessed by real-time, reverse transcription-polymerase chain reaction (RT-PCR) in HepG2 cells. In addition, HepG2 proliferation assay was performed in all groups. Notch1 and its target genes (Hes1 and cyclin D1) were downregulated in all treated groups with more suppressive effect in the groups treated with both MSCs and NCD. Also, treated HepG2 cells showed significant decrease in cell proliferation rate. These data suggest that modulation of Notch1 signaling pathway by MSCs and/or NCD can be considered as a therapeutic target in HCC.
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spelling pubmed-37601792013-09-10 Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line Abdel Aziz, Mohamed Talaat Khaled, Hussien Mostafa El Hindawi, Ali Roshdy, Nagwa Kamal Rashed, Laila A. Sabry, Dina Hassouna, Amira A. Taha, Fatma Ali, Walaa Ibrahim Biomed Res Int Research Article This study was conducted to evaluate the effect of mesenchymal stem cells (MSCs) and a novel curcumin derivative (NCD) on HepG2 cells (hepatoma cell line) and to investigate their effect on Notch1 signaling pathway target genes. HepG2 cells were divided into HepG2 control group, HepG2 cells treated with MSC conditioned medium (MSCs CM), HepG2 cells treated with a NCD, HepG2 cells treated with MSCs CM and NCD, and HepG2 cells treated with MSCs CM (CM of MSCs pretreated with a NCD). Expression of Notch1, Hes1, VEGF, and cyclin D1 was assessed by real-time, reverse transcription-polymerase chain reaction (RT-PCR) in HepG2 cells. In addition, HepG2 proliferation assay was performed in all groups. Notch1 and its target genes (Hes1 and cyclin D1) were downregulated in all treated groups with more suppressive effect in the groups treated with both MSCs and NCD. Also, treated HepG2 cells showed significant decrease in cell proliferation rate. These data suggest that modulation of Notch1 signaling pathway by MSCs and/or NCD can be considered as a therapeutic target in HCC. Hindawi Publishing Corporation 2013 2013-08-19 /pmc/articles/PMC3760179/ /pubmed/24024180 http://dx.doi.org/10.1155/2013/129629 Text en Copyright © 2013 Mohamed Talaat Abdel Aziz et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Abdel Aziz, Mohamed Talaat
Khaled, Hussien Mostafa
El Hindawi, Ali
Roshdy, Nagwa Kamal
Rashed, Laila A.
Sabry, Dina
Hassouna, Amira A.
Taha, Fatma
Ali, Walaa Ibrahim
Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line
title Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line
title_full Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line
title_fullStr Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line
title_full_unstemmed Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line
title_short Effect of Mesenchymal Stem Cells and a Novel Curcumin Derivative on Notch1 Signaling in Hepatoma Cell Line
title_sort effect of mesenchymal stem cells and a novel curcumin derivative on notch1 signaling in hepatoma cell line
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760179/
https://www.ncbi.nlm.nih.gov/pubmed/24024180
http://dx.doi.org/10.1155/2013/129629
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